菜豆黄转绿突变体ytg-2叶色变异机制及突变基因的初步定位  

Mechanism of Leaf Color Variation and Initial Localization of ytg-2 Mutant in Yellow-to-green Beans(Phaseolus vulgaris L.)

作　　者：王仔艳 黄誉程 冯国军[1] 杨晓旭 刘大军[1] 闫志山[1] 刘畅[1] WANG Ziyan;HUANG Yucheng;FENG Guojun;YANG Xiaoxu;LIU Dajun;YAN Zhishan;LIU Chang(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150000,Heilongjiang,China)

机构地区：[1]黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150000

出　　处：《中国蔬菜》2025年第4期87-96,共10页China Vegetables

基　　金：黑龙江省重点研发计划项目(SC2022ZX02C0202);农业部政府购买合同项目(19240656)。

摘　　要：植物叶色黄转绿突变易于识别,可作为标记性状应用于生产中的种子纯度鉴定、良种繁育、杂种纯度鉴定及杂交种生产。以菜豆叶片黄转绿突变体ytg-2及其野生型A18为试验材料,对其农艺性状、光合特性、叶绿素合成中间代谢物质含量以及叶绿体超微结构进行测定分析。结果表明:与野生型A18相比,突变体ytg-2苗期叶片呈现黄色表型,叶绿素含量低,表现为总叶绿素缺失,主要是由于叶绿素合成过程中coprogenⅢ到protoⅨ的合成受阻导致。随着生长发育,叶片由黄色转变为浅绿色,总叶绿素含量也随之升高。遗传分析显示,突变体ytg-2叶片黄转绿表型是由单隐性核基因控制。BSA测序将突变基因初步定位于1号染色体,位于开始位置46290000 bp、结束位置51430000 bp的5.14 Mb区间内。研究结果为探究黄转绿突变体叶色变异的生理机制,定位与叶色黄转绿相关的基因奠定基础。The transition from yellow to green in plant leaf color is readily identifiable and serves as a marker characteristic in seed purity identification,seed breeding,hybrid purity identification,and hybrid production.This study utilized the bean leaf yellow-to-green mutant ytg-2 and its wild type A18 as experimental materials.An analysis was conducted on their agronomic traits,photosynthetic characteristics,chlorophyll synthesis intermediate metabolites content,and chloroplast ultrastructure.Compared to A18,the ytg-2 leaves at the seedling stage exhibited a yellow phenotype,characterized by low chlorophyll content and total chlorophyll loss.This was primarily attributed to the obstructed synthesis of coprogenⅢto protoⅨduring chlorophyll synthesis.As the growth process continued,the leaves transitioned from yellow to light green,and the total chlorophyll content increased.Genetic analysis revealed that the yellow-to-green phenotype of the ytg-2 mutant was governed by a single recessive nuclear gene.BSA sequencing initially positioned the mutant gene on chromosome 1,ranging from 46290000 bp to 51430000 bp within a 5.14 Mb area.This preliminary gene mapping lays the groundwork for investigating the physiological mechanism of leaf color variation in yellow-to-green mutants and mapping the genes associated with this color change.

关 键 词：菜豆 叶色突变体 黄转绿 基因定位 叶绿素 

分 类 号：S643.1[农业科学—蔬菜学]