香瓜茄茎段愈伤组织离体再生体系的建立  

Establishment of In Vitro Regeneration System for Callus Induction from Stem Segments of Solanum muricatum

作　　者：别赵微 詹得利 曹云河 吴雨江 王丽慧[1,2] 杨世鹏[1,2] BIE Zhaowei;ZHAN Deli;CAO Yunhe;WU Yujiang;WANG Lihui;YANG Shipeng(Qinghai Key Laboratory of Vegetable Genetics and Physiology,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China;Laboratory for Research and Utilization of Germplasm Resources in Qinghai Tibet Plateau,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农林科学院,青海省蔬菜遗传与生理重点实验室,青海西宁810016 [2]青海大学农林科学院,青藏高原种质资源研究与利用重点实验室,青海西宁810016

出　　处：《中国蔬菜》2025年第4期116-125,共10页China Vegetables

基　　金：国家重点研发计划项目(2022YFD1602400);青海省乡村振兴科技示范县专项(2023-NK-X01)。

摘　　要：以青海地区香瓜茄(人参果)栽培品种青灿香脱毒苗为试验材料,探究不同消毒处理对香瓜茄茎段外植体的消毒效果以及不同激素配比对愈伤组织形成、不定芽和不定根分化的影响。结果表明,0.1%升汞处理香瓜茄茎段外植体8 min,污染率和死亡率最低;香瓜茄茎段试管苗快繁时最适合使用不含外源激素的MS培养基;诱导愈伤组织形成的最佳培养基为MS+0.5 mg·L^(-1)6-BA+1 mg·L^(-1)NAA或MS+2 mg·L^(-1)6-BA+2 mg·L^(-1)KT,诱导率与愈伤组织量都较高;愈伤组织不定芽分化最佳培养基为MS+2 mg·L^(-1)6-BA+1 mg·L^(-1)KT,分化率达98.50%,共获取180株再生苗母株(愈伤组织未继代),后续经快繁处理,再生苗与母株皆未出现显著的形态变异;诱导不定根分化的最佳培养基为MS+0.5 mg·L^(-1)IBA。研究结果为香瓜茄主栽品种青灿香离体再生和遗传转化体系的构建提供了技术支持。Using virus-free seedlings of‘Qingcanxiang’,a cultivated variety of Solanum muricatum in the Qinghai region,as experimental material,this study explored the disinfection effects of different sterilization treatments on Solanum muricatum stem explants and the influence of different hormone ratios on callus formation,adventitious bud and adventitious root differentiation.The results showed that treating explants with 0.1%mercuric chloride for 8-10 minutes resulted in the lowest contamination and mortality rates.For rapid propagation of Solanum muricatum stem segments in test tubes,MS medium without exogenous hormones was most suitable.The optimal media for inducing callus formation were MS+0.5 mg·L^(-1)6-BA+1 mg·L^(-1)NAA or MS+2 mg·L^(-1)6-BA+2 mg·L^(-1)KT,with induction rates and callus volume both relatively high.The optimal medium for adventitious bud differentiation from callus was MS+2 mg·L^(-1)6-BA+1 mg·L^(-1)KT,with a differentiation rate of 98.50%,yielding a total of 180 regenerated mother plants,without callus subculture.Subsequently,after rapid propagation treatment,neither the regenerated seedlings nor the mother plants showed significant morphological variations.Comprehensively considered,the optimal medium for inducing adventitious root differentiation was MS+0.5 mg·L-1 IBA.This research provides technical support for the establishment of in vitro regeneration and genetic transformation systems for‘Qingcanxiang’,the main cultivated variety of Solanum muricatum.

关 键 词：香瓜茄 人参果 茎段 愈伤组织 离体再生 

分 类 号：S642.9[农业科学—蔬菜学]