Evaluation of bio-efficacy of Metarhizium anisopliae against the pink bollworm Pectinophora gossypiella(Saunders),with insights into its colonization potential and insecticide compatibility  

作　　者：THAVKAR Snehal THUBE Shivaji PANCHBHAI Pramod LAVHE Nandkishor PILLAI Tinni SHAH Vivek DESHMUKH Vrushali PANDIAN Thavaprakasa R. FAND Babasaheb TENGURI Prabhulinga NIKOSHE Akash PRIYANK Mhatre BEHERE Gajanan PRASAD Yenumula 

机构地区：[1]College of Agriculture,Nagpur,Maharashtra 440001,India [2]Indian Council of Agricultural Research(ICAR)-Central Institute for Cotton Research,Nagpur,Maharashtra 440010,India [3]ICAR-Central Plantation Crops Research Institute,Regional Station,Vittal,Karnataka 574243,India [4]ICAR-Central Potato Research Institute Regional Station,Tamil Nadu,Udhagamandalam 643004,India [5]Divi-sion of Crop Protection,ICAR-Central Institute for Cotton Research,Nagpur,440010,India

出　　处：《Journal of Cotton Research》2025年第1期76-88,共13页棉花研究(英文)

摘　　要：Background The pink bollworm(Pectinophora gossypiella,PBW)is a major cotton pest,causing economic losses by damaging seeds and fiber.Cotton growers typically use systemic and broad-spectrum insecticides for its manage-ment,which pose risks to human health and the environment.Consequently,there is a need for eco-friendly alterna-tives.This study evaluates the bio-efficacy of the entomopathogenic fungus Metarhizium anisopliae strain TMBMA1 against pink bollworm and assesses its compatibility with major insecticides.Additionally,to comprehend the dynam-ics of colonization and the infection processes of entomopathogenic fungi(EPF),scanning electron microscopy(SEM)of infected larvae was carried out.Result We challenged the second instar PBW larvae to eight different concentrations(1×10^(3) to 1×10^(10) conidia mL^(-1))of an M.anisopliae strain TMBMA1.The highest mortality(100%)occurred at the higher concentrations i.e.,1×109 and 1×10^(10) spores mL^(-1),while the lowest mortality rate(46.6%)was observed at 1×10^(3) spores mL^(-1) con-centration compared to control(3.33%).TMBMA1’s biocontrol efficacy was validated by Probit analysis,exhibiting an exceptionally low median lethal concentration(LC50)value of 7.1×10^(5).The comparative evaluation revealed that the M.anisopliae strain TMBMA1 performed excellently with insecticide[Cypermethrin 10%(volume fraction)emulsifiable concentrate(EC)at 1 mL·L^(-1) water]giving 100%mortality,both being superior to a commercial prod-uct of M.anisopliae(60%).According to SEM analysis,the EPF strain was profusely colonized on both the internal and external surfaces of PBW larvae.Compatibility studies with insecticides revealed>98%and>96%reduction in the sporulation of M.anisopliae due to the treatment of Emamectin Benzoate 1.5%(mass fraction)+Profenofos 35%(mass fraction)water dispersible granules(WDG)and Profenofos 50%EC,respectively.In contrast,Cypermethrin 10%EC,Emamectin Benzoate 5%(mass fraction)Soluble Granules and Neem Seed Kernel Extract(NSKE)0.15%(volume fraction)treatm

关 键 词：Bio-efficacy Compatibility Entomopathogenic fungi Metarhizium anisopliae Pink bollworm SEM 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]