罗丹明B荧光法检测湖水中铅离子  

Rhodamine B fluorescence method for detecting lead ions in lake water

作　　者：贾超凡 张凤娇 徐新秋 闫业浩 魏胤 张璟 JIA Chaofan;ZHANG Fengjiao;XU Xinqiu;YAN Yehao;WEI Yin;ZHANG Jing(School of Public Health,Binzhou Medical University,Yantai 264000,China;School of Public Health,Jining Medical University,Jining 272013,China;School of Public Health,Shandong First Medical University,Jinan 250000,China)

机构地区：[1]滨州医学院公共卫生学院,烟台264000 [2]济宁医学院公共卫生学院,济宁272013 [3]山东第一医科大学公共卫生学院,济南250000

出　　处：《济宁医学院学报》2025年第2期125-128,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金青年项目(ZR2020QH297)。

摘　　要：目的利用罗丹明B荧光淬灭高灵敏度检测湖水中铅离子(Pb^(2+))。方法基于配位作用,罗丹明B与Pb^(2+)结合后,在577 nm处的荧光强度显著降低。通过优化实验条件,记录荧光强度变化,建立荧光强度与Pb^(2+)浓度的线性关系,并评价其在实际样品检测中的准确性和可靠性。结果Pb^(2+)在45~55μg/mL浓度范围内与荧光强度呈良好的线性关系,线性方程为y=-25.8761x+3265.9653,相关系数R 2为0.9746,检出限为1μg/mL。选择性试验表明,罗丹明B对Pb^(2+)具有较高的选择性,其他常见金属离子(如K+、Mg^(2+)、Mn^(2+)、Na+、Zn^(2+)等)对检测结果的干扰较小。在45μg/mL和55μg/mL 2个浓度水平下,平均回收率为93.40%~99.60%,相对标准偏差为0.99%~2.33%。结论该方法操作便捷,样品处理简单,试剂用量少,选择性好,灵敏度高,能够快速、准确地检测湖水中的Pb^(2+),具有良好的实际应用潜力。Objective To develop a highly sensitive detection method for lead ions(Pb^(2+))in lake water using Rhodamine B fluorescence quenching.Methods Based on coordination interactions,the fluorescence intensity of Rhodamine B at 577nm significantly decreases upon binding with Pb^(2+).By optimizing experimental conditions and recording fluorescence intensity changes,a linear relationship between fluorescence intensity and Pb^(2+)concentration was established.The accuracy and reliability of the method for real sample detection were also evaluated.Results A good linear relationship between Pb^(2+)concentration and fluorescence intensity was observed in the range of 45~55μg/mL,with a linear equation of y=-25.8761x+3265.9653 and a correlation coefficient R 2=0.9746.The detection limit was 1μg/mL.Selectivity tests showed that Rhodamine B exhibited high selectivity for Pb^(2+),with minimal interference from common metal ions(eg,K+,Mg^(2+),Mn^(2+),Na+,Zn^(2+)).At spiked concentrations of 45μg/mL and 55μg/mL,the average recovery rate ranged from 93.40%to 99.60%,with relative standard deviations of 0.99%-2.33%.Conclusion This method is operationally simple,requires minimal sample preparation and reagent usage,and demonstrates excellent selectivity and sensitivity.It enables rapid and accurate detection of Pb^(2+)in lake water and shows strong potential for practical applications.

关 键 词：罗丹明B 荧光法 铅离子 

分 类 号：O652[理学—分析化学]