三草保肝汤抑制MDSCs抗肝纤维化作用研究  

Study on the anti-hepatic fibrosis effect of Sancao Baogan decoction by inhibiting MDSCs

作　　者：沈立都 代鑫燕 吉胡日布 张诗盈 郭嘉诚 姜慧玲 严亨秀[1] SHEN Lidu;DAI Xinyan;JIHU Ribu;ZHANG Shiying;GUO Jiacheng;JIANG Huiling;YAN Hengxiu(School of Pharmacy and Food,Southwest Minzu University,Chengdu 610041,China;School of Grassland Resources,Southwest Minzu University,Chengdu 610225,China)

机构地区：[1]西南民族大学药学与食品学院,四川成都610041 [2]西南民族大学草地资源学院,四川成都610225

出　　处：《西南民族大学学报(自然科学版)》2025年第2期166-176,共11页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省科技厅应用基础项目(2021YJ0256);西南民族大学中央高校基本科研业务费专项资金优秀学生培养工程项目(2023NYXXS086,ZYN2024075)。

摘　　要：旨在探讨三草保肝汤(Sancao Baogan decoction,SBD)对CCl_(4)诱导小鼠肝纤维化的治疗作用及可能机制.小鼠按4 mL/kg腹腔注射5%CCl_(4)溶液诱导肝纤维化.自造模之日起,用SBD按高、低剂量连续灌胃给药6周,每天1次.通过HE、微板法、免疫组化、比色法、TUNEL法、ELISA、流式细胞术等检测SBD对肝纤维化的抑制作用及对MDSCs、T淋巴细胞和相关细胞因子的影响.结果显示,SBD组小鼠肝组织病理损伤、炎症浸润及胶原沉积得到明显改善;小鼠血清中AST和ALT活性显著下降;肝脏中MDA含量及α-SMA、IL-1β、IL-6、TGF-β1水平下调;肝脏和血液中MDSCs的表达明显下降;HSCs被显著抑制,肝细胞凋亡减少;GSH含量、CD4^(+)T淋巴细胞和CD8^(+)T淋巴细胞表达以及抗炎因子IL-10水平上调.以上结果表明,SBD对小鼠肝纤维化有较好的保护作用,并可能通过抑制肝脏MDSCs的免疫抑制作用提高小鼠免疫力进而减轻肝纤维化.This study investigated the intervention effect of Sancao Baogan decoction(SBD)on CCl_4-induced hepatic fibrosis in mice.The mouse hepatic fibrosis model was prepared by intraperitoneal injection of 5% CCl_(4)solution at a dose of 4 mL/kg.Commencing from the moldeling date,SBD was administered via continuous intragastric delivery at high or low doses once a day for 6 weeks.The inhibitory effect of SBD on hepatic fibrosis and its effect on MDSCs,T-lymphocytes and related cytokines were detected by methods including HE,microplate assay,immunohistochemistry,colorimetric assay,TUNEL assay,ELISA and flow cytometry.The results showed that the pathological injury,inflammatory infiltration and collagen deposition of liver tissue in SBD groups were significantly improved.The activities of AST and ALT in mouse serum exhibited a significant reduction.The MDA content and levels of α-SMA,IL-1β,IL-6,and TGF-β1 in the liver were significantly down-regulated.The expression of MDSCs in liver and blood was significantly decreased.HSCs was significantly inhibited and hepatocyte apoptosis was reduced.Whereas the expression of GSH,CD4^(+) T lymphocytes and CD8^(+) T lymphocytes,as well as the anti-inflammatory factor IL-10 were upregulated.The above results indicate that SBD has a good protective effect against hepatic fibrosis in mice,and the mechanism may be related to improving the immunity of mice by inhibiting the immunosuppressive effect of hepatic MDSCs and thus alleviating hepatic fibrosis.

关 键 词：三草保肝汤(SBD) MDSCS 肝纤维化 免疫调节 

分 类 号：R285[医药卫生—中药学]