Alowpathogenic avian influenzaA/Mallard/South Korea/KNU2019-34/2019(H1N1)virus has the potential to increase the mammalian pathogenicity  

作　　者：Jaemoo Kim Jungho Kim Suhyeon Heo Chang-Hun Yeom Bao Tuan Duong Haan Woo Sung Seon-Ju Yeo Hyun Park Haryoung Poo Jihyun Yang 

机构地区：[1]Center for Study of Emerging and Re-emerging Viruses,Korea Virus Research Institute,Institute for Basic Science(IBS),Daejeon 34126,Republic of Korea [2]Department of Biomedical Science and Engineering,Konkuk University,Seoul 05029,Republic of Korea [3]Infectious Disease Research Center,Korea Research Institute of Bioscience and Biotechnology(KRIBB),Daejeon 34141,Republic of Korea [4]Zoonosis Research Center,Department of Infection Biology,School of Medicine,Wonkwang University,Iksan 54651,Republic of Korea [5]College of Veterinary Medicine,Kangwon National University,Chuncheon 200-70124341,Republic of Korea [6]Department of Tropical Medicine and Parasitology,Department of Biomedical Sciences,College of Medicine,Seoul National University,Seoul 08826,Republic of Korea [7]Institute of Endemic Diseases,Medical Research Center,Seoul National University,Seoul 08826,Republic of Korea

出　　处：《Virologica Sinica》2025年第1期24-34,共11页中国病毒学(英文版)

基　　金：funded by grants from the National Research Foundation of Korea(NRF)grant funded by the Korea government(2018M3A9H4055203 and 2023R1A2C2003679);from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI),funded by the Ministry of Health&Welfare,Republic of Korea(HV23C1857);from KRIBB Research Initiative Program(KGM9942421).

摘　　要：Influenza,a highly contagious respiratory infectious disease caused by an influenza virus,is a threat to public health worldwide.Avian influenza viruses(AIVs)have the potential to cause the next pandemic by crossing the species barrier through mutation of viral genome.Here,we investigated the pathogenicity of AIVs obtained from South Korea and Mongolia during 2018–2019 by measuring viral titers in the lungs and extrapulmonary organs of mouse models.In addition,we assessed the pathogenicity of AIVs in ferret models.Moreover,we compared the ability of viruses to replicate in mammalian cells,as well as the receptor-binding preferences of AIV isolates.Genetic analyses were finally performed to identify the genetic relationships and amino acid substitutions between viral proteins during mammalian adaptation.Of the 24 AIV isolates tested,A/Mallard/South Korea/KNU2019-34/2019(KNU19-34;H1N1)caused severe bodyweight loss and high mortality in mice.The virus replicated in the lungs,kidneys,and heart.Importantly,KNU19-34-infected ferrets showed high viral loads in both nasal washes and lungs.KNU19-34 replicated rapidly in A549 and bound preferentially to human likeα2,6-linked sialic acids rather than to avian-likeα2,3-linked sialic acids,similar to the pandemic A/California/04/2009(H1N1)strain.Gene segments of KNU19-34 were distributed in Egypt and Asia lineages from 2015 to 2018,and the virus had several amino acid substitutions compared to H1N1 AIV isolates that were non-pathogenic in mice.Collectively,the data suggest that KNU19-34 has zoonotic potential and the possibility of new mutations responsible for mammalian adaptation.

关 键 词：Avian influenza virus(AIV) H1N1 Zoonotic potential Mutation Receptor binding specificity FERRET 

分 类 号：R373[医药卫生—病原生物学] S852.65[医药卫生—基础医学]