Diagnostic Efficiency of Tongue-Coating Microbiome in Patients With SARS-CoV-2 Omicron Variant Infection and Recovery  

作　　者：Junyi Sun Ying Sun Yawen Zou Haiyu Wang Benchen Rao Xinyue Zhang Liwen Liu Guizhen Zhang Guangying Cui Qi Liu Jiyuan Xing Ning Xu Chunfu Zheng Zujiang Yu Zhigang Ren 

机构地区：[1]Department of Infectious Diseases,State Key Laboratory of Antiviral Drugs,Pingyuan Laboratory,the First Affiliated Hospital of Zhengzhou University,Zhengzhou,China [2]Emergency Intensive Care Ward,the First Affiliated Hospital of Zhengzhou University,Zhengzhou,China [3]Department of Stomatology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou,China

出　　处：《Infectious Microbes & Diseases》2025年第1期54-64,共11页感染微生物与疾病(英文)

基　　金：supported by grants from the National Key Research and Development Program of China(2022YFC2303100);the Henan Province Epidemic Prevention and Control Emergency Scientific Research Project(221111311700 and 221111311600);the Independent and Innovation Project for Graduate Students of Zhengzhou University(20230444);Funding for Scientific Research and Innovation Team of The First Affiliated Hospital of Zhengzhou University(QNCXTD2023002).

摘　　要：SARS-CoV-2 variants still pose threats to human public health.However,there has been little research regarding alterations in the tongue-coating microbiome in patients infected with the Omicron variant(PIOVs).Herein,we collected 963 tongue-coating samples prospectively,including 349 samples from PIOVs,242 samples from recovered PIOVs,300 samples from healthy controls(HCs)and 72 samples from patients infected with the original strain(PIOSs).We randomly selected tongue-coating samples from PIOVs and HCs as the discovery cohort and validation cohort.Tongue-coating microbiota was analyzed using Miseq sequencing.Our results showed that the tongue-coating microbial diversity of PIOVs was increased.We found that in PIOVs,the abundance was increased in 20 genera,including Prevonella and Atopobium,while the abundance was decreased in 23 genera,including Neisseria and Haemoph-ilus.The classifier based on six optimal microbial markers had high diagnostic efficiency in the discovery cohort(area under the curve of 97.73%)and the validation cohort(area under the curve of 93.06%)between the PIOV and HC groups.Importantly,compared with PIOSs,PIOVs showed an increase in Fusobacterium.Recovery of patients was associated with the restoration of the tongue-coating microbiota.In conclusion,this study is the first to characterize the tongue-coating microbiota in PIOVs and to construct noninvasive diagnostic models,providing new strategies for the prevention and control of coronavirus variants.

关 键 词：COVID-19 SARS-CoV-2 Omicron variant recovery patients tongue-coating microbiome 

分 类 号：R563.1[医药卫生—呼吸系统]