Molecular Pathotyping and Phylogeny of Recent Subgenotype XIV.2 Newcastle Disease Viruses From Chickens and Pigeons in Nigeria  

作　　者：Bala Ningi Umar Jibril Adamu Paul A.Abdu Kabiru H.Ahmad Aliyu Sada Haruna M.Kazeem 

机构地区：[1]Department of Veterinary Microbiology,Faculty of Veterinary Medicine,Ahmadu Bello University,Zaria,Nigeria [2]Department of Veterinary Medicine,Faculty of Veterinary Medicine,Ahmadu Bello University,Zaria,Nigeria [3]Central Diagnostic Unit,National Veterinary Research Institute(NVRI),Vom,Nigeria.

出　　处：《Infectious Microbes & Diseases》2025年第1期65-69,共5页感染微生物与疾病(英文)

摘　　要：Genomic studies of Newcastle disease virus(NDV)affecting chickens and pigeons in Nigeria are limited.This study characterized NDVs from chickens and pigeons within households and live bird markets in Bauchi State,Nigeria.A total of 80 pooled oropharyngeal and cloacal swab samples were collected from November 2021 to February 2022 and tested using one-step RT-PCR.Interestingly,73(91.25%)of the samples were found to be positive,and 40 sequences of the partial NDV F gene were obtained.The fusion protein cleav-age site amino acid motifs 112R-R-Q/R-K-R-F117 and 112G-R-Q-G-R-L117 were observed for the virulent(95.0%)and lentogenic(5.0%)pathotypes,respectively.The virulent NDV strains clustered with subgenotype XIV.2 NDV strains from Nigeria and neighboring Benin,whereas the lentogenic pathotypes belonged to the genotype II cluster along with NDV vaccine strains.This study highlighted the se-rious threat of virulent NDV,warranting surveillance and improved biosecurity to control local and transboundary spread of NDV.

关 键 词：avian orthoavulavirus-1 Newcastle disease virus molecular characterization Orthoavulavirus javaense live bird market local chickens PIGEONS NIGERIA 

分 类 号：S852.65[农业科学—基础兽医学]