50日龄中华鲟幼鱼肠道微生物群落结构特征及其影响因素  

Analysis of intestinal microbial community structure of 50 DAH Acipenser sinensis juvenile and its impact factors

作　　者：田甜 张建明 朱欣 张德志 胡亚成 TIAN Tian;ZHANG Jianming;ZHU Xin;ZHANG Dezhi;HU Yacheng(Chinese Sturgeon Research Institute of China Three Gorges Corporation/Hubei Key Laboratory of Three Gorges Project for Fish Resource Conservation,Yichang 443100,China)

机构地区：[1]中国长江三峡集团有限公司中华鲟研究所/三峡工程鱼类资源保护湖北省重点实验室,湖北宜昌443100

出　　处：《南方水产科学》2025年第2期102-109,共8页South China Fisheries Science

基　　金：湖北省自然科学基金面上项目(2023AFB989);中国长江三峡集团有限公司自主科研项目(WWKY-2020-0079)。

摘　　要：肠道菌群被称作宿主的“第二基因组”。探究外界因素对中华鲟(Acipenser sinensis)幼鱼肠道微生物群落结构的影响,对中华鲟幼鱼的健康培育具有重要参考价值。以50日龄中华鲟幼鱼为研究对象,基于高通量测序技术分析了肠道和肠道内容物的菌群特征,并研究了菌群结构与饵料、养殖水源水之间的相关性。结果显示,在属分类水平上,肠道和肠道内容物之间的微生物组成存在差异,肠道优势菌属为不动杆菌属(Acinetobacter)、乳球菌属(Lactococcus)、柠檬酸杆菌属(Citrobacter)、假单胞菌属(Pseudomonas)和Clostridium_sensu_stricto_1,而肠道内容物优势菌属为乳球菌属、Clostridium_sensu_stricto_1和土孢杆菌属(Terrisporobacter)。通过SourceTracker物种来源分析追踪微生物的来源,结果显示,肠道微生物中有82.68%来源于饵料,1.89%来源于养殖水源水;肠道内容物微生物中有94.60%来源于饵料,0.93%来源于养殖水源水。研究表明,中华鲟幼鱼肠道和肠道内容物的微生物组成在属分类水平上存在差异,前者优势菌属多为潜在致病菌,后者多为有益菌;与养殖水源水相比,饵料对肠道和肠道内容物的微生物群落组成具有更高的贡献。Intestinal microbiome is regarded as the"second genome"of host.Comprehending the influences of external factors on the structure of the intestinal microbial community in juvenile Chinese sturgeon(Acipenser sinensis)can provide a reference for its healthy breeding.Taking 50 DAH A.sinensis juvenile as objects,we applied the high-throughput sequencing technology to analyze the characteristics of intestine and intestinal content,and explore their correlation with feed and culture source water.The results show that on genus level,there were differences in the microbial composition between intestine and intestinal con-tent.The intestine was mainly composed of Acinetobacter,Lactococcus,Citrobacter,Pseudomonas and Clostridium_sensu_stricto_1,while the intestinal content was mainly composed of Lactococcus,Clostridium_sensu_stricto_1 and Terrisporobacter.Source Tracker analysis verified that 82.68%of the intestine community came from the feed,and 1.89%came from the culture source water.While 94.60%of the intestinal content community came from the feed,and 0.93%came from the culture source water.The study indicates that there are differences in the microbial composition of the intestine and intestinal content of the ju-venile on genus level.The dominant bacterial genera in the former are mostly potential pathogenic bacteria,while those in the latter are mostly beneficial bacteria.Compared with the culture source water,the feed has a higher contribution to the composi-tion of the intestine and intestinal content microbial community.

关 键 词：中华鲟幼鱼 肠道 肠道内容物 微生物群落 饵料 养殖水源水 

分 类 号：S943[农业科学—水产养殖]