多线盘鮈人工繁殖和胚胎发育规律研究  

Study on artificial propagation and embryonic development regularity of Discogobio multilineatus

作　　者：王岳松 杨洋 徐林 甘星 何大江 程睿 黎国樑 毕建启 陈锋[1] 张志明 WANG Yuesong;YANG Yang;XU Lin;GAN Xing;HE Dajiang;CHENG Rui;LI Guoliang;BI Jianqi;CHEN Feng;ZHANG Zhiming(Institute of Hydroecology,Ministry of Water Resources and Chinese Academy of Sciences/Key Laboratory of Ecological Impacts of Hydraulic Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources,Wuhan 430079,China;Guangxi Datengxia Gorge Water Conservancy Development Co.Ltd.,Nanning 530000,China;Innovation Team of the Changjiang Water Resources Commission for River and Lake Ecosystem Restoration Key Technology,Wuhan 430079,China)

机构地区：[1]水利部中国科学院水工程生态研究所/水利部水工程生态效应与生态修复重点实验室,湖北武汉430079 [2]广西大藤峡水利枢纽开发有限责任公司,广西南宁530000 [3]长江水利委员会流域河湖生态系统修复关键技术创新团队,湖北武汉430079

出　　处：《南方水产科学》2025年第2期157-163,共7页South China Fisheries Science

基　　金：科技基础资源调查专项(2019FY1011900);来宾市红水河珍稀鱼类增殖保护站运行管理(DHB2022013)。

摘　　要：多线盘鮈(Discogobio multilineatus)为红水河特有鱼类,分布区域狭窄,野生资源匮乏。为实现多线盘鮈苗种的规模化生产,为其人工增殖放流提供技术支撑,对人工驯养的多线盘鮈亲鱼进行强化培育和催产,通过干法授精获得受精卵,并观察其胚胎发育过程。研究显示,多线盘鮈绝对繁殖力为(415.53±133.27)粒、相对繁殖力为(24.24±5.64)粒·g^(−1)。共进行3次人工繁殖,平均催产率为(93.12±1.73)%、受精率为(67.03±3.62)%、孵化率为(61.34±2.13)%。多线盘鮈受精卵为沉性卵,无黏性,呈淡青色或浅黄色,成熟卵子直径为(2.11±0.04)mm,吸水膨胀后呈白色,卵膜径达(3.37±0.09)mm。水温22~24℃下,经67~91 h孵化出膜,孵化积温为1728.17 h·℃,历经胚盘期、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和孵化期共7个发育阶段、26个时期。初孵仔鱼全长为(6.25±0.17)mm,肌节26对。Discogobio multilineatus is a species endemic to the Hongshui River,with a narrow distribution range and scarce wild resources.In order to achieve the mass production of its larvae and provide technical support for its enhancement and release,the artificially bred D.multilineatus was intensively cultured and artificially induced to spawn.The fertilized eggs were obtained through dry fertilization,and the embryonic development process was observed.The results show that the absolute fecundity of D.multilineatus was(415.53±133.27)grains,and the relative fecundity was(18.70±4.67)grains·g^(-1).A total of three times of artificial breeding were conducted,with average spawning rate of(93.12±1.73)%,fertilization rate of(67.03±3.62)%,and hatching rate of(61.34±2.13)%.The fertilized eggs of D.multilineatus were demersal,non-adhesive,in light blue or pale yellow color,with an eggshell diameter of(2.11±0.04)mm.After absorbing water and swelling,they turned white,with an egg membrane diameter of(3.37±0.09)mm.At water temperatures of 22−24℃,the eggs hatched and eclosed after 67−91 h,with a total hatching and eclosion energy of 1728.17℃·h.The embryonic development went through seven stages and 26 periods,including the blastula stage,cleavage stage,blastocyst stage,gastrula stage,neural plate stage,organogenesis stage,and hatching stage.The newly hatched larvae had a total length of(6.25±0.17)mm and 26 myotomes.

关 键 词：多线盘鮈 人工繁殖 胚胎发育 红水河 

分 类 号：S961.2[农业科学—水产养殖]