半夏凝集素的分离纯化及其对肝癌细胞增殖的影响  

Isolation and purification of Pinellia ternata lectin and its effect on the proliferation of liver cancer cells

作　　者：方炯浩 古贞伟 何慧 何畹莹 余雅莹 邹小燕 周林 FANG Jionghao;GU Zhenwei;HE Hui;HE Wanying;YU Yaying;ZOU Xiaoyan;ZHOU Lin(Guangdong Provincial Key Laboratory for Research and Evaluation of Pharmaceutical Preparations/School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Dreampharm Biotechnology Co.,Ltd.,Guangzhou 510300,China)

机构地区：[1]广东省药物制剂研究与评价重点实验室/广东药科大学生命科学与生物制药学院,广东广州510006 [2]广东隽沐生物科技有限公司,广东广州510300

出　　处：《广东药科大学学报》2025年第2期93-98,共6页Journal of Guangdong Pharmaceutical University

基　　金：2022年广东省教育厅研究生教育创新计划项目(51315073010)。

摘　　要：目的分离纯化半夏蛋白中的半夏凝集素(pinellia ternata lectin,PTL),进行鉴定及细胞活性分析。方法采用制备型高效液相色谱(PHPLC)对PTL进行分离纯化,结合质谱、糖蛋白检测、凝血活性以及生物活性分析等方法进行鉴定。结果在流速为6 mL/min、洗脱剂为40%乙腈的条件下,通过PHPLC分离得到12 kDa的目标蛋白,制备效率为60%。经过质谱及凝血活性分析,确定其为PTL。高碘酸-希夫染色法检测其为糖蛋白,且对小鼠红细胞具有凝集活性。20~140μg/mL的PTL能够显著促进HepG2和Hepa1-6肝癌细胞的增殖。结论成功从半夏中分离鉴定出PTL,并发现一定质量浓度的PTL能促进肿瘤细胞的增殖。Objective To isolate and purify Pinellia ternata lectin(PTL)from Pinellia ternata protein,and to perform identification and cellular activity analysis.Methods PTL was isolated and purified by preparative highperformance liquid chromatography(PHPLC),combined with mass spectrometry,glycoprotein detection,coagulation activity,and biological activity analysis.Results At a flow rate of 6 mL/min and an eluent of 40%acetonitrile,a 12 kDa target protein was isolated by PHPLC with a preparation efficiency of 60%.Mass spectrometry and coagulation activity analysis confirmed it as PTL.Periodic acid-Schiff staining detected it as a glycoprotein with hemagglutination activity against mouse red blood cells.PTL at concentrations of 20-140μg/mL significantly promoted the proliferation of HepG2 and Hepa1-6 liver cancer cells.Conclusion PTL has been successfully isolated and identified from Pinellia ternata,and it has been found that PTL at certain concentrations can promote the proliferation of tumor cells.

关 键 词：半夏凝集素 分离纯化 凝血活性 细胞增殖 

分 类 号：R932[医药卫生—生药学]