液相色谱-串联质谱法同时检测尿液和血液中8种合成大麻素及7种代谢物  

Simultaneous Determination of 8 Synthetic Cannabinoids and 7 Metabolites in Urine and Blood by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：杨思瑶 何洪源[1] 蒋力维 吕建霞 滕傲雪 YANG Si-yao;HE Hong-yuan;JIANG Li-wei;LÜJian-xia;TENG Ao-xue(School of Criminal Investigation,People’s Public Security University of China,Beijing 100038,China;National Narcotics Laboratory Beijing Regional Center,Beijing 100164,China;Criminal Crime Investigation Bureau of Chengdu Public Security Bureau,Chengdu 610000,China)

机构地区：[1]中国人民公安大学侦查学院,北京100038 [2]国家毒品实验室北京分中心,北京100164 [3]成都市公安局刑事犯罪侦查局,四川成都610000

出　　处：《分析测试学报》2025年第4期583-592,共10页Journal of Instrumental Analysis

基　　金：中国人民公安大学刑事科学技术双一流创新研究专项(2023SYL06);北京市科技计划资助项目(Z221100005222006)。

摘　　要：建立了液相色谱-串联质谱(LC-MS/MS)对尿液和血液中8种合成大麻素及7种代谢物进行定性和定量分析的方法。尿液、血液样品经甲醇-乙腈(体积比1∶1)混合溶液提取后,高速离心取上清液,以0.1%甲酸水溶液和乙腈作为流动相进行梯度洗脱,采用C18色谱柱进行分离,电喷雾电离源(ESI+)多反应监测模式(MRM)进行检测,可在15 min内完成15种目标物的分析。结果显示,尿液中15种目标物在1~200 ng/mL范围内线性关系良好,检出限为0.01~0.10 ng/mL,定量下限为0.03~0.34 ng/mL;血液中15种目标物在0.50~200 ng/mL范围内线性关系良好,检出限为0.01~0.20 ng/mL,定量下限为0.03~0.66 ng/mL。3个不同加标浓度下,15种目标物在尿液、血液中的提取回收率分别为92.2%~105%和82.2%~117%。该方法灵敏度高、分离效果好、操作简便,适用于尿液和血液中多种合成大麻素类物质的定性定量分析。A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the qualitative and quantitative analysis of eight synthetic cannabinoids and seven metabolites in urine and blood.After the urine and blood samples were extracted by a mixture of methanol-acetonitrile(1∶1,volume ratio),the supernatant was extracted by high-speed centrifugation,and the gradient elution was carried out with an aqueous solution containing 0.1%formic acid and acetonitrile as the mobile phase,and the separation was carried out on a C18 chromatography column.The ion source for the tandem mass spectrometry was an electrospray ionization source(ESI+),and the detection was performed in multiple reaction monitoring(MRM)mode.The method can be used for the analysis of 15 targets within 15 min.The results showed that the 15 targets exhibited a good linear relationship in the range of 1-200 ng/mL in urine,the limits of detection(LODs)were 0.01-0.10 ng/mL,and the limits of quantification(LOQs)were 0.03-0.34 ng/mL.The 15 targets exhibited a good linear relationship in the range of 0.50-200 ng/mL in blood,LODs were 0.01-0.20 ng/mL,and LOQs were 0.03-0.66 ng/mL.The recoveries in urine and blood at three different spiked concentrations were 92.2%-105%and 82.2%-117%.The developed method is sensitive,effective in separation,easy to operate,and suitable for the determination analysis of synthetic cannabinoid analogs in urine and blood.

关 键 词：合成大麻素 代谢物 液相色谱-串联质谱 血液 尿液 

分 类 号：O657.7[理学—分析化学] D918.93[理学—化学]