废弃黑木耳菌糠多糖的提取工艺优化  

Optimization of Extraction Process of Polysaccharides from Waste Black Fungus Bran

作　　者：褚盼盼[1] 刘英洁 刘承葳 候恬靖 CHU Panpan;LIU Yingjie;LIU Chengwei;HOU Tianjing(Lyuliang University Department of Biological and Food Engineering,Lyuliang,Shanxi 033000,China)

机构地区：[1]吕梁学院生物与食品工程系,山西吕梁033000

出　　处：《天津农业科学》2025年第1期1-11,共11页Tianjin Agricultural Sciences

基　　金：吕梁市科技计划项目(2023NYYF16)。

摘　　要：为优化废弃黑木耳菌糠多糖提取工艺,分别将黑木耳菌糠和多糖得率作为材料和指标,采用热水浸提法和酶辅助提取法提取黑木耳菌糠多糖,通过单因素试验和响应面法优化,以期得到黑木耳菌糠多糖最佳提取工艺。结果表明,热水浸提法的最佳工艺条件为料液比1∶14、提取时间3 h、提取温度81℃,多糖得率为5.251%,影响因素大小排序为C(提取温度)>A(料液比)>B(提取时间);酶辅助提取法的最佳工艺条件为pH值4.4、料液比1∶15、提取温度56℃、提取时间4 h,多糖得率为13.642%,影响因素大小排序为A(pH)>D(提取时间)>C(提取温度)>B(料液比);经比较,以黑木耳菌糠多糖得率为指标,酶辅助提取法提取多糖明显优于热水浸提法。综上,从黑木耳菌糠中提取多糖的方法可行,为废弃食用菌菌糠的资源化利用指明方向。In order to optimize the extraction conditions of polysaccharides from waste auricularia auricularia bran,the auricularia auricularia bran was used as the material and the yield of polysaccharide was used as the index.Hot water extraction method and enzyme-assisted extraction method were used to extract polysaccharides from auricularia auricularia bran,and the optimal extraction process of polysaccharide from auricularia auricularia bran was obtained by single factor test and response surface method.The results showed that the optimal process conditions of hot water extraction were as follows:solid-liquid ratio 1:14,extraction time 3 h,temperature 81℃,so that polysaccharide yield was 5.251%.The importance order of influencing factors was C(extraction temperature)>A(solid-liquid ratio)>B(extraction time).The optimal conditions of enzyme-assisted extraction were:pH value 4.4,solid-liquid ratio 1∶15,temperature 56℃,time 4 h,so that polysaccharide yield was 13.642%.The importance order of influencing factors was A(pH)>D(time)>C(extraction temperature)>B(solid-liquid ratio).The results showed that the enzyme-assisted extraction method was superior to the hot water extraction method in extracting polysaccharides from fungus bran.In conclusion,it is feasible to extract polysaccharides from black fungus bran,which points out the direction of resource utilization of waste edible fungus bran.

关 键 词：黑木耳菌糠 多糖得率 热水浸提法 酶辅助提取法 提取工艺 

分 类 号：S646.6[农业科学—蔬菜学]