上调miR-152抑制ox-LDL诱导的人血管平滑肌细胞增殖和迁移  

Upregulation of miR-152 inhibits proliferation and migration of human vascular smooth muscle cells induced by ox-LDL

作　　者：周晶 屈苗 ZHOU Jing;QU Miao(Function Department,Baoji Central Hospital,Baoji 721008,China)

机构地区：[1]宝鸡市中心医院功能科,陕西宝鸡721008

出　　处：《基础医学与临床》2025年第4期486-492,共7页Basic and Clinical Medicine

摘　　要：目的研究miR-152和Rho相关卷曲螺旋蛋白激酶1(ROCK1)在氧化型低密度脂蛋白(ox-LDL)干预的血管平滑肌细胞(VSMCs)中的作用。方法通过ox-LDL干预人血管平滑肌细胞CRL-1999建立体外动脉粥样硬化模型,转染miR-152 mimic和ROCK1 siRNA至对应细胞,RT-qPCR检测miR-152和ROCK1 mRNA表达,CCK-8法检测细胞增殖活性,Trasnwell实验检测细胞迁移能力,荧光素酶报告基因检测miR-152和ROCK1的靶向关系。结果以100μg/mL ox-LDL处理48 h的CRL-1999细胞增殖率最大;与未被ox-LDL未处理的对照组相比较,ox-LDL诱导组的miR-152相对表达显著降低(P<0.05);转染miR-152 mimic后显著降低了CRL-1999细胞增殖活性和迁移能力(P<0.05);荧光素酶报告基因测定显示miR-152靶向调节ROCK1,miR-152 mimic转染的CRL-1999细胞中ROCK1 mRNA和蛋白质表达降低(P<0.05);转染ROCK1 siRNA显著降低了CRL-1999细胞增殖活性和迁移能力(P<0.05)。结论miR-152通过下调ROCK1的表达,抑制了ox-LDL诱导的CRL-1999细胞增殖和迁移,这可能是动脉粥样硬化的潜在治疗靶点。Objective To investigate the roles of miR-152 and Rho-associated coiled-coil kinase 1(ROCK1)in vascular smooth muscle cells(VSMCs)treated with oxidized low-density lipoprotein(ox-LDL).Methods Human vascular smooth muscle cells CRL-1999 were intervened with ox-LDL to establish an in vitro atherosclerosis model.miR-152 mimic and ROCK1 siRNA were transfected into corresponding cells.RT-qPCR was used to detect the expression of miR-152 and ROCK1 mRNA.The CCK-8 method was used to assess cell proliferation activity,and the Transwell assay was used to test cell migration ability.A luciferase reporter gene assay was conducted to determine the targeting relationship between miR-152 and ROCK1.Results CRL-1999 cells treated with 100μg/mL ox-LDL for 48 hours showed the maximum proliferation rate.Compared with the untreated control group,the relative expression of miR-152 in the ox-LDL-induced group was significantly decreased(P<0.05).Transfection of miR-152 mimic significantly reduced the proliferation and migration of CRL-1999 cells(P<0.05).The luciferase reporter gene assay indicated that miR-152 targets ROCK1,and expression of ROCK1 mRNA and protein in miR-152 mimic group was reduced(P<0.05).Transfection of ROCK1 siRNA also significantly decreased the proliferation and migration of CRL-1999 cells(P<0.05).Conclusions miR-152 inhibits the ox-LDL induced proliferation and migration of CRL-1999 cells by downregulating ROCK1 expression,which may be a potential therapeutic target for atherosclerosis.

关 键 词：miR-152 动脉粥样硬化 Rho相关卷曲螺旋蛋白激酶1 血管平滑肌 增殖 转移 

分 类 号：R587.1[医药卫生—内分泌]