精子DNA碎片指数与冷冻损伤易感性、精液常规及胚胎质量的关系研究  

The correlation between sperm DNA fragmentation index and susceptibility to cryodamage,routine semen parameters and embryo quality

作　　者：张伊慧 胡梦琳[1] 徐泽胜 练易初 倪吴花[1,3] ZHANG Yi-hui;HU Meng-lin;XU Ze-sheng;LIAN Yi-chu;NI Wu-hua(Center of Reproductive Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000;Central Laboratory,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000;Wenzhou Key Laboratory of Reproduction&Genetics,Wenzhou 325000)

机构地区：[1]温州医科大学附属第一医院生殖医学中心,温州325000 [2]温州医科大学附属第一医院中心实验室,温州325000 [3]温州市生殖遗传重点实验室,温州325000

出　　处：《生殖医学杂志》2025年第4期456-461,共6页Journal of Reproductive Medicine

基　　金：国家自然科学青年基金(81903293);温州市生殖遗传重点实验室项目(2022HZSY0051)。

摘　　要：目的探讨精子冷冻保存对精子DNA碎片化指数(DFI)的影响以及精子DFI与常规精液分析和胚胎质量的可能关系。方法利用精子染色质结构分析(SCSA)和精子染色质扩散试验(SCD)方法检测104例男性不育门诊患者精子DFI,比较两种方法的一致性。同时,比较精液冷冻前后精子DFI的变化,并分析DFI与精液常规和胚胎质量的可能关系。结果SCSA与SCD法对精子DFI的检测结果具有显著的线性正相关性(R 2=0.809,P<0.0001)。常规冷冻后精子DFI显著增加(P<0.0001),其中高DFI组(DFI>15%)精子冷冻后的DFI差值高于低DFI组(DFI≤15%)[5.5(1.9,14.6)%vs.4.9(2.3,8.2)%],但无显著差异(P>0.05)。高DFI组精子浓度和精子活力显著低于低DFI组(P<0.05),但高、低DFI组间胚胎质量比较无显著差异(P>0.05)。结论SCSA法和SCD法检测DFI有较好的一致性;高DFI组精子浓度和精子活力显著降低;冷冻增高精子DFI,并且可能对原本高DFI精子影响更大。Objective:To investigate the effects of sperm cryopreservation on sperm DNA fragmentation index(DFI)and the correlations between DFI and routine semen parameters,embryo quality.Methods:Sperm chromatin structure assay(SCSA)and sperm chromatin dispersion(SCD)were used to determine sperm DFI of 104 male infertility outpatients,and the concordance between these two methods was also compared.In addition,the changes in sperm DFI before and after sperm cryopreservation were compared.The correlation between DFI and routine sperm parameters and embryo quality were also analyzed.Results:There was a significant linear positive correlation between SCSA and SCD in sperm DFI analysis(R 2=0.809,P<0.0001).Sperm DFI significantly increased after standard cryopreservation(P<0.0001),and the DFI increase post-cryopreservation was greater in the high DFI group(DFI>15%)compared with the low DFI group(DFI≤15%),but without a significant difference(P>0.05).Sperm concentration and motility were significantly lower in the high DFI group(P<0.05),while there was no significant difference in embryo quality between two DFI groups(P>0.05).Conclusions:The SCSA and SCD methods were consistent in determining sperm DFI.Sperm concentration and motility were lower in the high DFI group.Cryopreservation increased sperm DFI and had a greater impact on sperm with high DFI.

关 键 词：男性不育 精子冷冻保存 精子DNA碎片 精子染色质结构分析 染色质扩散试验 

分 类 号：R711.6[医药卫生—妇产科学]