2024年河南省3例输入性D8基因型麻疹病例的病原学特征分析  

Molecular epidemiology of three imported D8 genotype measles cases in Henan province in 2024

作　　者：唐玉婷 丰达星[2] 王文慧 张延炀[2] 路明霞[2] 肖占沛[2] 白祎然 张燕[3] 王慧玲[3] 豆巧华 刘倩[2] 孔江南 赵玉玲[2] 周慧萍 刘迎迎 王小丽 郭永豪 Tang Yuting;Feng Daxing;Wang Wenhui;Zhang Yanyang;Lu Mingxia;Xiao Zhanpei;Bai Yiran;Zhang Yan;Wang Huiling;Dou Qiaohua;Liu Qian;Kong Jiangnan;Zhao Yuling;Zhou Huiping;Liu Yingying;Wang Xiaoli;Guo Yonghao(College of Public Health,Zhengzhou University,Zhengzhou 450001,Henan,China;Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,Henan,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Zhengzhou Center for Disease Control and Prevention,Zhengzhou 450012,Henan,China)

机构地区：[1]郑州大学公共卫生学院,河南郑州450001 [2]河南省疾病预防控制中心,河南郑州450016 [3]中国疾病预防控制中心病毒病预防控制所,北京102206 [4]郑州市疾病预防控制中心,河南郑州450012

出　　处：《中国疫苗和免疫》2025年第1期62-67,共6页Chinese Journal of Vaccines and Immunization

摘　　要：目的分析河南省2024年3例输入性麻疹病例麻疹病毒(Measles virus,MV)分离株病原学特征。方法开展麻疹病例流行病学调查,采用酶联免疫吸附试验检测血清MV IgM抗体,采用荧光定量聚合酶链反应检测咽拭子MV核酸,对核酸阳性标本进行MV细胞培养。采用逆转录聚合酶链反应扩增MV分离株序列,构建与参考株序列的亲缘性关系树,分析氨基酸和核苷酸序列一致性以及氨基酸变异。结果3例麻疹病例包括儿童1例、成人2例,从其他国家或中国其他省份旅居河南省;且MV IgM抗体、核酸检测均阳性,其中1例细胞培养阳性。3株MV分离株与D8基因型参考株MVi/Manchester.GBR/30.94/D8属于同一个分支,分别与D8基因型命名株MVs/Rudaki.TJK/49.21、MVs/Almaty.KAZ/10.23、MVs/Patan.IND/16.19的氨基酸和核苷酸序列一致性最高,分别为100%和99.8%、100%和100%、100%和100%。3株分离株序列与D8基因型参考株、中国疫苗株序列分别存在3~4个、16~19个氨基酸变异位点。结论本研究输入性麻疹病例MV分离株均为D8基因型,且存在一定变异。需继续加强流行病学和病原学监测,及时识别和处置输入性麻疹疫情。Objective To analyze genetic sequences of measles virus(MV)isolates from three imported measles cases in Henan province in 2024.Methods We conducted an epidemiological investigation of measles cases,tested sera for MV IgM antibody using enzyme-linked immunosorbent assay,tested throat swabs for MV nucleic acid using fluorescence quantitative polymerase chain reaction,and cultured nucleic acid positive isolates.We amplified the sequences of MV isolates using reverse transcription polymerase chain reaction and constructed a phylogenetic tree compared with reference strains to determine amino acid and nucleotide sequence consistency and amino acid variation.Results The three measles cases included one child and two adults who traveled from other countries or another province of China to Henan province.All cases were positive for MV IgM antibody and nucleic acid;one case was able to be cell cultured.The three MV isolates belonged to the D8 genotype reference strain MVi/Manchester.GBR/30.94/D8,with high amino acid and nucleotide sequence consistencies of 100%and 99.8%,100%and 100%,and 100%and 100%,compared with D8 genotype strains MVs/Rudaki.TJK/49.21,MVs/Almaty.KAZ/10.23,and MVs/Patan.IND/16.19,respectively.The three isolates had 3-4 amino acid differences from the D8 genotype reference strain and 16-19 amino acid differences from the Chinese vaccine strain.Conclusions The MV isolates from imported measles cases were D8 genotype with some variation.It is necessary to strengthen epidemiological and etiological monitoring for rapid identification and responses of imported measles cases.

关 键 词：麻疹 麻疹病毒 D8基因型 输入性病例 病原学特征 

分 类 号：R186[医药卫生—流行病学] R512.62[医药卫生—公共卫生与预防医学]