银杏叶提取物银杏内酯延缓人骨关节炎进展的体外作用研究  

Effect of Ginkgolide on Delaying the Progression of Human Osteoarthritis in Vitro

作　　者：洪厚松 贺小楚 郭炼锦 HONG Housong;HE Xiaochu;GUO Lianjin(The Fourth Affiliated Hospital of Guangzhou Medical University,Guangzhou City,Guangdong Province 511300)

机构地区：[1]广州医科大学附属第四医院,广东省广州市511300

出　　处：《医学理论与实践》2025年第8期1261-1264,共4页The Journal of Medical Theory and Practice

摘　　要：目的:观察银杏叶提取物银杏内酯(GIN)对人骨关节炎软骨细胞(HC-OA)增殖、凋亡基因及炎症因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的影响。方法:选取生长状态良好的HC-OA,并用二甲基亚砜(DMSO)溶解配置不同浓度GIN,备用。首先检测GIN对HC-OA细胞增殖能力以及细胞凋亡的影响,按GIN浓度的不同将实验依次分为空白对照(二甲基亚砜,DMSO)组、GIN(5μmol/L)组、GIN(10μmol/L)组、GIN(100μmol/L)组。空白对照组常规培养液培养,各组加入相应浓度GIN处理后,应用CCK-8法检测细胞增殖能力变化,实时定量PCR检测细胞凋亡基因BCL2/BAX/Caspase-3表达情况。另外,检测GIN对HC-OA细胞炎性因子表达情况的影响,按处理方法不同将实验分为空白对照(DMSO)组、GIN(100μmol/L)组、脂多糖(LPS)(10μg/mL)组以及GIN(100μmol/L)+LPS(10μg/mL)组,应用酶联免疫吸附法检测GIN以及LPS处理后细胞上清炎症因子IL-1β、TNF-α水平。结果:GIN处理48h后,相比空白对照组,不同浓度的GIN均能显著促进HC-OA增殖,且呈浓度依赖性(P均<0.05);相比空白对照组,GIN(10μmol/L)组、GIN(100μmol/L)组处理48h后,凋亡相关基因BAX、Caspase-3表达下调,抗凋亡基因BCL-2表达显著上调(P均<0.05);相比空白对照组,LPS诱导后细胞上清炎症因子IL-1β、TNF-α水平显著上调;相比LPS组,LPS+GIN组HC-OA细胞上清炎症因子IL-1β、TNF-α水平显著下调,各组间两两比较差异均有统计学意义(P均<0.05)。结论:GIN体外能有效促进人骨关节炎软骨细胞增殖、抑制软骨细胞凋亡及软骨细胞中炎症因子IL-1β、TNF-α的表达。Objective:To investigate the effects of ginkgolide(GIN)from Ginkgo biloba leaf on proliferation and apoptosis genes and the expression of inflammatory factors interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in human osteoarthritis chondrocytes(HC-OA).Methods:HC-OA cells in healthy growth condition were selected,and GIN was dissolved in dimethyl sulfoxide(DMSO)to prepare solutions of varying concentrations for subsequent experiments.To evaluate the effects of GIN on HC-OA cell proliferation and apoptosis,the cells were divided into four experimental groups[blank control group(dimethyl sulfoxide,DMSO),GIN(5μmol/L)group,GIN(10μmol/L)group,GIN(100μmol/L)group]based on GIN concentration.The blank control group was maintained in standard culture medium,and all experimental groups were treated with their respective GIN concentrations.Changes in cell proliferation were assessed using the CCK-8 assay,and the expression levels of apoptosis-related genes BCL2/BAX/Caspase-3 were quantified by real-time quantitative PCR.To investigate the effects of GIN on inflammatory cytokine expression in HC-OA cells,the experiment was divided into four treatment groups:blank control(DMSO)group,GIN(100μmol/L)group,LPS(10μg/mL)group,and GIN(100μmol/L)+LPS(100μmol/L)group.Levels of IL-1βand TNF-αin cell supernatants were measured by ELISA following respective treatments.Results:48 hours after GIN treatment,compared with blank control group,different concentration of GIN could significantly promote the proliferation of HC-OA in a concentration-dependent manner(all P<0.05).Compared with blank control group,the expressions of apoptosis-related genes BAX and Caspase-3 in GIN(10μmol/L)group and GIN(100μmol/L)group were down-regulated,and the expression of anti-apoptosis-related genes BCL-2 was significantly up-regulated after 48 hours of treatment(P<0.05).Compared with the control group,the levels of inflammatory cytokines IL-1βand TNF-αwere significantly up-regulated after induction by LPS.Compared with LPS group,the leve

关 键 词：银杏内酯 骨关节炎 软骨细胞 增殖 凋亡 

分 类 号：R684.3[医药卫生—骨科学]