大豆TPS基因家族在盐胁迫下的表达变化及单倍型选择规律分析  

Changes in the Expression of the Soybean TPS Gene Family Under Salt Stress and Haplotype Selection Analysis

作　　者：曹婕 卢秋连 翟健平 刘宝辉 方超 李世晨 苏彤 Jie Cao;Qiulian Lu;Jianping Zhai;Baohui Liu;Chao Fang;Shichen Li;Tong Su(Innovative Research Center of Molecular Genetics and Evolution/College of Life Sciences,Guangzhou University,Guangzhou 510006,China;Guangdong Key Laboratory of Plant Adaptation and Molecular Design,Guangzhou 510006,China)

机构地区：[1]广州大学生命科学学院/分子遗传与进化创新研究中心,广州510006 [2]广东省植物适应性与分子设计重点实验室,广州510006

出　　处：《植物学报》2025年第2期172-185,共14页Chinese Bulletin of Botany

基　　金：广东省自然科学基金面上项目(No.2023A1515011668);黑龙江省自然科学基金(No.LH2021C078);河北省现代种业科技创新专项(No.22326316D)。

摘　　要：海藻糖-6-磷酸合酶(trehalose-6-phosphate synthase,TPS)是合成海藻糖的关键酶,已在多个物种中被报道参与调节光合作用、糖代谢、生长发育和逆境响应等生理过程。目前,TPS在大豆(Glycine max)中的报道极少。该文在大豆全基因组中鉴定了20个TPS基因及其包含的10种重要蛋白保守基序。启动子元件分析显示,大豆TPS基因的启动子区富含大量胁迫响应元件;盐胁迫处理后,17个TPS基因的表达发生变化,其中12个基因上调表达,5个基因下调表达。对TPS进行单倍型和选择趋势分析,发现TPS8、TPS13、TPS15、TPS17和TPS18存在2种主要的等位变异,其中携带TPS15^(H2)、TPS13^(H2)、TPS17^(H2)和TPS18^(H2)的品种在栽培品种中大量富集,受到强烈的人工选择。该研究揭示了大豆TPS基因家族的分子特征以及在盐胁迫下的表达模式和进化历史,旨在为进一步解析大豆TPS基因的功能以及培育耐盐大豆品种提供理论依据和遗传材料。INTRODUCTION:Trehalose-6-phosphate synthase(TPS)is a key enzyme involved in the synthesis of trehalose and has been reported to participate in regulating photosynthesis,carbohydrate metabolism,growth and development,and stress responses in various species.Currently,reports on TPS genes in soybean are scarce.RATIONALE:TPS is a stable non-reducing disaccharide,whose synthesis,decomposition and regulation not only provide energy for plant,but also play an important role in plant growth and development and stress tolerance.The in-depth study of soybean TPS genes and its relationships with salt stress is of great significance in elucidating the molecular mechanism of soybean salt tolerance and improving soybean yield.RESULTS:This study identified 20 soybean TPS genes and their associated 10 conserved protein motifs in the soybean genome.Molecular analysis of the promoter elements revealed that the TPS gene promoters are rich in stress-responsive elements.After salt stress treatment,the expression of 17 TPS genes changed,with 12 genes up-regulated and 5 genes down-regulated.Haplotype and selection analyses revealed two major allelic variations in TPS8,TPS13,TPS15,TPS17,and TPS18.Notably,variants carrying TPS15^(H2),TPS13^(H2),TPS17^(H2),and TPS18^(H2)were significantly enriched in improved cultivars that underwent strong artificial selection.CONCLUSION:This study reveals the molecular characteristics of the soybean TPS gene family,their expression patterns under salt stress,and their evolutionary history,providing a theoretical basis and genetic material for further elucidating the functions of soybean TPS genes and breeding salt-tolerant soybean varieties.

关 键 词：大豆 海藻糖-6-磷酸合酶 表达分析 耐盐 人工选择 

分 类 号：S565.1[农业科学—作物学]