番茄SlWRKY45转录因子在响应低温和干旱胁迫中的功能  

Functions of SlWRKY45 in Response to Low-temperature and Drought Stress in Tomato

作　　者：樊蓓 任敏 王延峰[1,2] 党峰峰[1,2] 陈国梁[1,2] 程国亭 杨金雨 孙会茹 Bei Fan;Min Ren;Yanfeng Wang;Fengfeng Dang;Guoliang Chen;Guoting Cheng;Jinyu Yang;Huiru Sun(College of Life Sciences,Yan’an University,Yan’an 716000,China;Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau,College of Life Sciences,Yan’an University,Yan’an 716000,China)

机构地区：[1]延安大学生命科学学院,延安716000 [2]延安大学生命科学学院,陕西省黄土高原资源植物研究与利用省市共建重点实验室,延安716000

出　　处：《植物学报》2025年第2期186-203,共18页Chinese Bulletin of Botany

基　　金：延安大学博士科研启动项目(No.YDBK2019-42);陕西省自然科学基础研究计划(No.2022JQ-159);陕西省大学生创新创业训练计划(No.S202310719073)。

摘　　要：番茄(Solanum lycopersicum)在生长发育过程中常受到低温和干旱等多种非生物胁迫的影响。WRKY转录因子参与调控植物多种非生物胁迫响应过程,而SlWRKY45在番茄非生物胁迫中的功能尚不清楚。基因表达分析发现,低温、干旱和ABA处理均可显著诱导SlWRKY45的表达;过表达SlWRKY45可提高番茄对干旱和低温的耐受性;在干旱和低温处理下,过表达株系的光合指标、抗氧化酶活性和脯氨酸(Pro)含量显著高于野生型(WT),活性氧(ROS)和丙二醛(MDA)含量显著低于WT。转录组数据分析显示,SlWRKY45主要通过调控抗氧化酶活性和胁迫响应途径介导番茄对低温胁迫的响应。双荧光素酶报告基因检测发现,SlWRKY45可直接激活SlPOD1的表达。酵母双杂交(Y2H)和双分子荧光互补(BiFC)试验结果表明,SlWRKY45与SlWRKY46存在相互作用。综上表明,SlWRKY45可能通过直接调控抗氧化酶途径增强转基因番茄的抗逆性,为番茄的遗传改良提供了重要的候选基因资源。INTRODUCTION:Tomato(Solanum lycopersicum),a significant warm-season and water-dependent vegetable crop,is extensively cultivated worldwide.Whether grown in open fields or protected environments,tomatoes frequently encounter various environmental stresses,including drought and low temperatures,which significantly impact their yield and quality.Transcription factors play a pivotal role in plant stress responses by modulating the expression of specific target genes,thereby transmitting perceived stress signals downstream.WRKY transcription factors in tomatoes are known to regulate responses to multiple abiotic stresses.However,the specific role of the tomato SlWRKY45 in abiotic stress responses remains unclear.RATIONALE:Studies have demonstrated that WRKY transcription factors play a crucial regulatory role in plant responses to abiotic stress.As an important economic vegetable crop,tomato is susceptible to various environmental stresses during its growth and development.By genetically overexpressing SlWRKY45 in tomato and investigating its function under low-temperature and drought stress conditions,the findings can provide a theoretical foundation for understanding the complex regulatory mechanisms of WRKY transcription factors.Additionally,this research offers valuable candidate genes for breeding stress-resistant tomato varieties.RESULTS:Expression analysis revealed that low-temperature,drought,and abscisic acid(ABA)treatments significantly induced the expression of SlWRKY45.Overexpression of SlWRKY45 enhanced the resistance of tomato plants to drought and low-temperature stresses.Under drought and low-temperature conditions,the photosynthetic indices,antioxidant enzyme activities,and proline(Pro)contents in SlWRKY45 overexpression lines were significantly higher than those in wild-type(WT)plants.Conversely,the accumulation of reactive oxygen species(ROS)and malondialdehyde(MDA)levels in SlWRKY45-OE plants was significantly lower than in WT plants under the same stress conditions.Transcriptome data analysis indi

关 键 词：番茄 SlWRKY45 干旱胁迫 低温胁迫 抗氧化酶 

分 类 号：S641.1[农业科学—蔬菜学]