基于纳米酶的纸基比色传感器在即时检测领域的应用研究进展  

Advances in Paper-based Colorimetric Sensors Based on Nanozymes for Point-of-Care Testing

作　　者：周双 梅雪娜 王少伟 杨红梅 于京华[1] 张彦 Shuang Zhou;Xuena Mei;Shaowei Wang;Hongmei Yang;Jinghua Yu;Yan Zhang(School of Chemistry and Chemical Engineering,University of Jinan,Jinan 250022,China;Shandong Provincial Yantai Ecological Environment Monitoring Center,Yantai 264000,China)

机构地区：[1]济南大学化学化工学院,济南250022 [2]山东省烟台生态环境监测中心,烟台264000

出　　处：《化学学报》2025年第2期170-183,共14页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.22306070,21904047);泰山学者工程(No.tsqn202103082);山东省高等学校青年创新团队发展计划(No.2021KJ021);中国博士后科学基金(Nos.2024T170344,2023M731302);山东省教育系统政府公派出国留学项目资助。

摘　　要：纳米酶,因兼具生物酶的良好催化活性和纳米材料结构特性,展现出稳定性高、活性可调控、环境耐受性好等优点,正逐渐成为生物酶的有效替代品.纳米酶优异的催化活性使其广泛应用于比色传感分析,结合纸基材成本低廉、孔隙率高和易生物降解等特点,近年来基于纳米酶的纸基比色传感器已成为一种简便有效的即时检测工具.本文首先阐述了纸基比色传感器的构造与工作原理;然后,对纳米酶的类型和催化反应机制进行了详细介绍;其次,回顾了基于纳米酶的纸基比色传感器在快速检测领域的主要应用实例,涵盖了食品安全分析、环境质量监测与生物医学诊断等重点领域;最后,探讨了目前基于纳米酶的纸基比色传感器在即时检测应用领域面临的挑战,并对其未来的发展潜力进行了展望.Nanozymes, characterized by their combination of the favorable catalytic activity of biological enzymes with the structural properties of nanomaterials, have emerged as effective substitutes for natural enzymes. They exhibit high stability, adjustable activity, and robust environmental tolerance, which makes them as ideal candidates for various applications. The exceptional catalytic properties have facilitated their widespread adoption in colorimetric sensing analysis, particularly when integrated with paper substrates. Paper, renowned for its low cost, high porosity, and biodegradability, serves as an excellent platform for developing simple and effective point-of-care testing (POCT) tools. In this paper, we first delve into the construction and operational principles of paper-based colorimetric sensors. These sensors leverage the unique characteristics of paper to create microfluidic channels and reaction zones, enabling the controlled flow and mixing of analytes with detection reagents. The colorimetric response, resulting from the catalytic reaction between the analyte and the nanozyme-based reagent, serves as the basis for qualitative and quantitative analysis. Subsequently, a comprehensive overview of the types of nanozymes and their catalytic reaction mechanisms is provided. Nanozymes encompass a wide range of materials, including metal oxides, metal sulfides, carbon-based nanomaterials, metal-organic frameworks, and single-atom nanozymes, etc., each exhibiting distinct catalytic properties tailored for specific sensing applications. The catalytic reactions involve the oxidation or reduction of analytes, leading to a color change that can be visually detected or analyzed using a smartphone or spectrophotometer. Furthermore, the significant applications of paper-based colorimetric sensors based on nanozymes for POCT are reviewed, focusing on key areas such as food safety analysis, environmental quality monitoring, and biomedical diagnosis. Despite lots of advantages, paper-based colorimetric sensors based

关 键 词：即时检测 纳米酶 纸基 比色 传感分析 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]