A low-cost printed circuit board-based centrifugal microfluidic platform for dielectrophoresis  

作　　者：Nicklas Rondot Songyuan Yan Dario Mager Lawrence Kulinsky 

机构地区：[1]Institute of Microstructure Technology,Karlsruhe Institute of Technology,Karlsruhe,Germany [2]Henry Samueli School of Engineering,University of California,Irvine,CA,USA

出　　处：《Microsystems & Nanoengineering》2025年第1期289-299,共11页微系统与纳米工程(英文)

摘　　要：In recent decades, electrokinetic handling of microparticles and biological cells found many applications ranging frombiomedical diagnostics to microscale assembly. The integration of electrokinetic handling such as dielectrophoresis(DEP) greatly benefits microfluidic point-of-care systems as many modern assays require cell handling. Compared totraditional pump-driven microfluidics, typically used for DEP applications, centrifugal CD microfluidics provides theability to consolidate various liquid handling tasks in self-contained discs under the control of a single motor.Therefore, it has significant advantages in terms of cost and reliability. However, to integrate DEP on a spinning disc, amajor obstacle is transferring power to the electrodes that generate DEP forces. Existing solutions for power transferlack portability and availability or introduce excessive complexity for DEP settings. We present a concept that leveragesthe compatibility of DEP and inductive power transfer to bring DEP onto a rotating disc without much circuitry. Oursolution leverages the ongoing advances in the printed circuit board market to make low-cost cartridges (<$1) thatcan employ DEP, which was validated using yeast cells. The resulting DEPDisc platform solves the challenge thatexisting printed circuit board electrodes are reliant on expensive high-voltage function generators by boosting thevoltage using resonant inductive power transfer. This work includes a device costing less than $100 and easilyreplicable with the information provided in the Supplementary material. Consequently, with DEPDisc we present thefirst DEP-based low-cost platform for cell handling where both the device and the cartridges are truly inexpensive.

关 键 词：centrifugal microfluidics dielectrophoresis cell handling microscale assembly centrifugal cd microfluidics biological cells electrokinetic handling consolidate various liquid handling tas 

分 类 号：TN41[电子电信—微电子学与固体电子学]