Fully automated in vivo screening system for multi-organ imaging and pharmaceutical evaluation  

作　　者：Junhan Duan Guanming Lin Kangjian Jiao Xiaohui Hong Xudong Lin 

机构地区：[1]Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument,School of Biomedical Engineering,Shenzhen Campus of Sun Yat-Sen University,518000 Shenzhen,China

出　　处：《Microsystems & Nanoengineering》2025年第1期301-311,共11页微系统与纳米工程(英文)

基　　金：supported by the National Natural Science Foundation of China(82372088);the National Natural Science Foundation of Guangdong Province(2021A1515010266);the Shenzhen Science and Technology Program(202206193000001,20220816161126002);the Foundation of Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument(2020B1212060077);2021 Foshan Science and Technology Innovation Team for Young-Top Talents(2120001010795).

摘　　要：Advancements in screening technologies employing small organisms have enabled deep profiling of compounds in vivo.However,current strategies for phenotyping of behaving animals,such as zebrafish,typically involve tedious manipulations.Here,we develop and validate a fully automated in vivo screening system(AISS)that integrates microfluidic technology and computer-vision-based control methods to enable rapid evaluation of biological responses of non-anesthetized zebrafish to molecular gradients.Via precise fluidic control,the AISS allows automatic loading,encapsulation,transportation and immobilization of single-larva in droplets for multi-organ imaging and chemical gradients generation inaccessible in previous systems.Using this platform,we examine the cardiac sensitivity of an antipsychotic drug with multiple concentration gradients,and reveal dramatic diversity and complexity in the accurate chemical regulation of cardiac functions in vivo.This proposed system expands the arsenal of tools available for in vivo screening and facilitates comprehensive profiling of pharmaceuticals.

关 键 词：small organisms automated vivo screening system screening technologies microfluidic technology deep profiling compounds precise flui evaluation biological responses automated vivo screening system aiss 

分 类 号：R965[医药卫生—药理学]