Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo  

作　　者：Xiangyu Guo Antian Zhao Youchao Zhang Huanyu Jiang Longhua Tang Bo Lu Yibin Ying Mingchuan Zhou 

机构地区：[1]Robotic Micro-nano Manipulation Lab,College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou,China [2]College of Optical Science and Engineering,Zhejiang University,Hangzhou,China [3]Robotics and Microsystems Center,School of Mechanical and Electric Engineering,Soochow University,Suzhou,China

出　　处：《Microsystems & Nanoengineering》2025年第1期403-415,共13页微系统与纳米工程(英文)

基　　金：support from the National Natural Science Foundation of China(32101626);the Shandong Province Key R&D Plan Project(2022LZGC020);the Natural Science Foundation of Jiangsu Province of China(BK20220490);the Fundamental Research Funds for the Central Universities under Grant No.226-2024-00227.

摘　　要：The microinjection of Zebrafish embryos is significant to life science and biomedical research.In this article,a novel automated system is developed for cell microinjection.A sophisticated microfluidic chip is designed to transport,hold,and inject cells continuously.For the first time,a microinjector with microforce perception is proposed and integrated within the enclosed microfluidic chip to judge whether cells have been successfully punctured.The deep learning model is employed to detect the yolk center of zebrafish embryos and locate the position of the injection needle within the yolk,which enables enhancing the precision of cell injection.A prototype is fabricated to achieve automatic batch microinjection.Experimental results demonstrated that the injection efficiency is about 20 seconds per cell.Cell puncture success rate and cell survival rate are 100%and 84%,respectively.Compared to manual operation,this proposed system improves cell operation efficiency and cell survival rate.The proposed microinjection system has the potential to greatly reduce the workload of the experimenters and shorten the relevant study period.

关 键 词：deep learning model cell microinjectiona detect yolk center microfluidic chip microforce perception cell batch microinjection zebrafish embryo automated system 

分 类 号：TP2[自动化与计算机技术—检测技术与自动化装置]