Transparent,flexible graphene–ITO-based neural microelectrodes for simultaneous electrophysiology recording and calcium imaging of intracortical neural activity in freely moving mice  

作　　者：Miao Yuan Fei Li Feng Xue Yang Wang Baoqiang Li Rongyu Tang Yijun Wang Guo-Qiang Bi Weihua Pei 

机构地区：[1]Laboratory of Solid-State Optoelectronics Information Technology,Institute of Semiconductors,Chinese Academy of Sciences,Beijing 100083,China [2]Institute of Semiconductors,University of Chinese Academy of Sciences,Beijing 10049,China [3]Interdisciplinary Center for Brain Information,the Brain Cognition and Brain Disease Institute,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [4]Shenzhen-Hong Kong Institute of Brain Science,Shenzhen 518055,China [5]Hefei National Research Center for Physical Sciences at the Microscale,University of Science and Technology of China,Hefei 230026,China [6]School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Microsystems & Nanoengineering》2025年第1期417-430,共14页微系统与纳米工程(英文)

基　　金：support of the National Key R&D Program of China(2022YFF1202303 and 2023YFF1203702);NSFC-Guangdong Joint Fund(U20A6005);the National Natural Science Foundation of China(62071447);STI2030-Major Projects(2021ZD0200100);the Science and Technology Innovation Committee of Shenzhen Municipality(JCYJ20220818101611024).

摘　　要：To understand the complex dynamics of neural activity in the brain across various temporal and spatial scales,it is crucial to record intracortical multimodal neural activity by combining electrophysiological recording and calcium imaging techniques.This poses significant constraints on the geometrical,mechanical,and optical properties of the electrodes.Here,transparent flexible graphene–ITO-based neural microelectrodes with small feature sizes are developed and validated for simultaneous electrophysiology recording and calcium imaging in the hippocampus of freely moving mice.A micro-etching technique and an oxygen plasma pre-treating method are introduced to facilitate large-area graphene transfer and establish stable low-impedance contacts between graphene and metals,leading to the batch production of high-quality microelectrodes with interconnect widths of 10μm and recording sites diameters of 20μm.These electrodes exhibit appropriate impedance and sufficient transparency in the field of view,enabling simultaneous recording of intracortical local field potentials and even action potentials along with calcium imaging in freely moving mice.Both types of electrophysiological signals are found to correlate with calcium activity.This proof-of-concept work demonstrates that transparent flexible graphene–ITO-based neural microelectrodes are promising tools for multimodal neuroscience research.

关 键 词：electrophysiology re flexible graphene neural activity neural microelectrodes ITO electrophysiological recording record intracortical multimodal neural activity calcium imaging techniquesthis 

分 类 号：R318[医药卫生—生物医学工程]