督脉电针联合跑台训练对脊髓损伤大鼠运动功能的影响  

Effects of Du Meridian Electroacupuncture Combined with Treadmill Training on Motor Function of Rats with Spinal Cord Injury

作　　者：程枚 田宇 彭京 余双奇 张学俊 丁凡 CHENG Mei;TIAN Yu;PENG Jing;YU Shuangqi;ZHANG Xuejun;DING Fan(Wuhan Pu Ren Hospital,Wuhan,Hubei 430081,China;Sports Medicine College,Wuhan Sports University,Wuhan,Hubei 430079,China;The Third Affiliated Hospital of Southern Medical University,Guangzhou,Guangdong 510630,China)

机构地区：[1]武汉市普仁医院,湖北武汉430081 [2]武汉体育学院运动医学院,湖北武汉430079 [3]南方医科大学附属第三医院,广东广州510630

出　　处：《康复学报》2025年第2期160-168,共9页Rehabilitation Medicine

基　　金：国家自然科学基金青年科学基金项目(81601934);湖北省卫生健康科研基金项目(WJ2021F015)。

摘　　要：目的探讨督脉电针联合跑台训练对脊髓损伤(SCI)大鼠运动功能的影响及其作用机制。方法选择50只健康雌性SPF级SD大鼠,按照随机数字法分为假手术组、模型组、跑台组、电针组和跑台+电针组,每组10只。假手术组仅切除T10椎板,不损伤脊髓,其余4组均参考Allen's法和NYU脊髓损伤打击法建立T10脊髓损伤模型。排除死亡和BBB运动功能量表评分>2分大鼠,最终每组纳入8只。假手术组和模型组自由饮食、活动,不进行任何干预。跑台组接受跑台训练,1次/d,30 min/次,5 d/周,持续4周。电针组接受电针刺激,穴位选择大椎穴(DU14)和命门穴(GV4),1次/d,20 min/次,5次/周,持续4周。跑台+电针组先接受跑台训练,再接受电针训练,训练方法同跑台组和电针组。分别于术前、术后采用BBB运动功能量表评估大鼠脊髓损伤后的运动功能恢复情况。术后,采用磁共振成像(MRI)检测脊髓损伤段恢复情况;采用免疫组化法检测脑源性神经营养因子(BDNF)、神经生长因子(NGF)蛋白表达水平;采用Western blot检测突触后致密区蛋白-95(PSD-95)、突触蛋白Ⅰ(SynapsinⅠ)蛋白表达水平。结果①BBB评分:与模型组同一时间点比较,跑台组、电针组、跑台+电针组术后2、3、4周BBB评分均明显升高,差异均具有统计学意义(P<0.05)。与跑台组、电针组同一时间点比较,跑台+电针组术后3、4周BBB评分均明显升高,差异具有统计学意义(P<0.05)。②脊髓损伤段恢复情况:与模型组比较,跑台组和电针组大鼠脊髓组织均明显恢复,炎症反应区域减小。与模型组、跑台组和电针组比较,跑台+电针组脊髓结构组织更完整,损伤范围缩小,高信号更低,炎症反应区域减小。③BDNF、NGF蛋白表达水平:与假手术组、模型组、跑台组和电针组比较,跑台+电针组BDNF蛋白表达水平均明显升高(P<0.05);与模型组比较,跑台+电针组NGF蛋白表达水平明显升高(P<0.05)。④PSObjective To explore the effects and underlying mechanism of Du meridian electroacupuncture combined with treadmill training on motor function of rats with spinal cord injury(SCI).Methods A total of 50 healthy female SPF-grade SD rats were randomly divided into sham operation group,model group,treadmill group,electroacupuncture group and treadmill+electroacupuncture group,with 10 rats in each group.In the sham group,the T10 laminectomy was performed without SCI;while in the other four groups,Allen's method and New York University(NYU)spinal cord injury impactor method were used to establish T10 SCI model.Rats with death or BBB motor function scale score>2 were excluded,and finally eight rats were included in each group.The sham operation group and the model group had free diet and activities without any intervention.The treadmill group received treadmill training,once a day,30 minutes a time,five days a week for four weeks.The electroacupuncture group received electroacupuncture stimulation at Dazhui(DU14)and Mingmen(GV4),once a day,20 minutes a time,five times a week for four weeks.The treadmill+electroacupuncture group received treadmill training followed by electroacupuncture stimulation,and the training methods were the same as those of the treadmill group and the electroacupuncture group.Before and after the operation,BBB scale was used to evaluate the recovery of motor function after SCI.After operation,magnetic resonance imaging(MRI)was used to detect the recovery of the SCI segment;immunohistochemistry was used to detect the protein expression levels of brain-derived neurotrophic factor(BDNF)and nerve growth factor(NGF);Western blot was used to detect the protein expression levels of postsynaptic density-95(PSD-95)and SynapsinⅠ.Results(1)BBB score:compared with the model group at the same time,BBB scores of the treadmill group,the electroacupuncture group and the treadmill+electroacupuncture group increased significantly at 2,3,4 weeks after operation,and the differences were statistically significant(P

关 键 词：脊髓损伤 督脉 电针 跑台训练 运动功能 大椎穴(DU14) 命门穴(GV4) 

分 类 号：R245[医药卫生—针灸推拿学]