圆叶葡萄Warden果皮微生物多样性分析  

Analysis of Microbial Community Diversity in Muscadine(Muscadinia rotundifolia Michx.)Warden Grape Skins

作　　者：孙大运 尹玲 郭泽西 黄羽 曲俊杰[1] SUN Dayun;YIN Ling;GUO Zexi;HUANG Yu;QU Junjie(Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Key Lab,Nanning,Guangxi 530007,China;Grape and Wine Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西壮族自治区农业科学院/广西作物遗传改良生物技术重点实验室,广西南宁530007 [2]广西壮族自治区农业科学院葡萄与葡萄酒研究所,广西南宁530007

出　　处：《热带农业科学》2025年第3期23-29,共7页Chinese Journal of Tropical Agriculture

基　　金：中央引导地方科技发展资金项目(No.桂科ZY21195039);广西科技基地和人才专项(No.桂科AD21220116);广西重点研发计划(No.桂科AB21076001)。

摘　　要：为了解圆叶葡萄(Muscadinia rotundifolia Michx)果皮微生物的组成,揭示果皮微生物对圆叶葡萄品性的影响,以16S和ITS测序技术检测了圆叶葡萄Warden果皮微生物的多样性。结果显示:细菌主要有变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌(Bacteroidetes)、浮霉菌(Planctomycetes)、酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria),优势细菌属有乳杆菌属(Lactobacillus)、埃希氏菌属—志贺氏杆菌属(Escherichia-Shigella)、柄杆菌属(Caulobacter)和普氏菌属9(Prevotella9);真菌主要为子囊菌门(Ascomycota)和担子菌门(Basidiomycota),优势属为青霉菌属(Penicillium)、曲霉菌属(Aspergillus)、红曲霉属(Monascus)、炭疽菌属(Colletotrichum)、Catenulostroma和土赤壳属(Ilyonectria)。研究表明,圆叶葡萄Warden果皮聚集大量的微生物群落,为揭示微生物对圆叶葡萄风味多样性、酿酒品质及抗病性的影响提供了参考依据。To determine the composition of pericarp microorganisms from the muscadine grape(Muscadinia rotundifolia Michx)and provide a theoretical basis for revealing the effects of microorganisms on the grape,this study used high-throughput se-quencing 16S and ITS to analyze the microbial diversity in the muscadine Warden skins.The results revealed that the domi-nant bacterial phyla were Proteobacteria,Firmicutes,Bacteroidetes,Planctomycetes,Acidobacteria,and Actinobacteria,and the dominant genera were Lactobacillus,Escherichia-Shigella,Caulobacter and Prevotella_9.For fungi,the dominant phyla were Ascomycota and Basidiomycota,and the dominant genera were Penicillium,Aspergillus,Monascus,Colletotrichum,Catenulostroma and Ilyonectria.The identification of these microbes provides a reference for understanding the influence of microbes on the unique flavor,wine quality,and disease resistance of muscadine grapes.

关 键 词：圆叶葡萄 果皮微生物 16S ITS 

分 类 号：S663.1[农业科学—果树学]