一株宁乡猪源乳杆菌的分离鉴定及生物学特性研究  

Isolation,Identification and Biological Characteristics of a Lactic Acid Bacteria from Ningxiang Pig

作　　者：向书涵 尹杰[1] 张海涵 贺喜[1,2,3] 宋泽和 XIANG Shuhan;YIN Jie;ZHANG Haihan;HE Xi;SONG Zehe(College of Animal Science and Technology,Hunan Agricultural University,Hunan Changsha 410128,China;Engineering Research Center of Feed Safety and Efficient Utilization of China Ministry of Education,Hunan Changsha 410128,China;Collaborative Innovation Center for the High-quality Production of Livestock and Poultry,Hunan Changsha 410128,China)

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]饲料安全与高效利用教育部工程研究中心,湖南长沙410128 [3]优质畜禽产品生产省部共建协同创新中心,湖南长沙410128

出　　处：《饲料工业》2025年第7期143-148,共6页Feed Industry

摘　　要：研究旨在筛选宁乡猪源的饲用益生菌添加剂菌株。通过MRS培养基从宁乡猪粪便中分离出一株乳杆菌,经16S rRNA鉴定、革兰氏染色后确认其为食淀粉乳杆菌,之后对其进行了生长曲线绘制、产酸试验、耐酸试验、耐胆盐试验、自聚集试验、冻干保护剂初步探索试验。结果表明:分离的食淀粉乳杆菌在培养到22 h时进入生长平台期,进入平台期后培养基的pH可降至4.17;分离的食淀粉乳杆菌具有良好的耐酸能力,在pH为3.0的MRS培养基中仍能进行生长;在含有0.15%猪胆盐的MRS培养基中培养4 h后菌体浓度大于含有0.30%猪胆盐的MRS培养基中的菌体浓度;同时分离菌还具有较好的自聚集能力。由此可得,分离所得食淀粉乳杆菌是一株具有良好耐酸、耐胆盐和自聚集能力的乳杆菌,可作为畜禽用微生态制剂进行后续研究。The aim of this experiment was to screen forage probiotic additive strains derived from Ningxiang pig.A strain of Lactobacillus spp.was isolated from Ningxiang pig feces by MRS medium.The strain was further confirmed as Lactobacillus amylovorus by 16S rRNA identification and Gram staining test.Then,the strain was subjected to growth curve determination,acid production test,acid resistance test,bile salt resistance test,self-aggregation test,and preliminary exploration of lyophilized protectants.The results showed that the Lactobacillus amylovorus starch-eating entered the growth plateau at 22 h,and the pH of the medium could be reduced to 4.17 after entering the plateau.The strain has strong acid resistance and can still grow in MRS medium at pH 3.0.Furthermore,when cultured in MRS medium containing 0.15%procine bile salts for 4 hours,the bacterial concentration exceeded that observed in MRS medium containing 0.30%porcine bile salts.Meanwhile,the isolated strain exhibited favorable auto-aggregation capability.These results indicate that the isolated Lactobacillus amylovorus strain demonstrates robust acid tolerance,bile salt resistance,and auto-aggregation capacity,making it a promising candidate for further research as a probiotic for livestock and poultry.

关 键 词：食淀粉乳杆菌 宁乡猪 分离鉴定 生物学特性 饲用益生菌添加剂 

分 类 号：S816.3[农业科学—饲料科学]