老鼠簕乙醇提取物化学成分的提取、分离与鉴定  

Extraction,Isolation and Identification of Chemical Constituents from Ethanol Extract of Acanthus ilicifolius

作　　者：陈子然 高苏蕊 简敏如 刘韶松[3] 李元跃[1,2] CHEN Ziran;GAO Surui;JIAN Minru;LIU Shaosong;LI Yuanyue(Fisheries College,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Ecoenvironment,Xiamen 361021,China;Plant Introduction&Quarantine and Plant Product Key Laboratory of Xiamen City,Xiamen Overseas Substropical Plant Introduction Garden,Xiamen 361002,China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021 [3]厦门华侨亚热带植物引种园,厦门市植物引种检疫与植物源产物重点实验室,福建厦门361002

出　　处：《集美大学学报(自然科学版)》2025年第2期121-127,共7页Journal of Jimei University:Natural Science

基　　金：福建省高校产学合作重大项目“滨海湿地生态修复技术红树林种植-养殖耦合系统的研发与示范”(2021Y4013);厦门华侨亚热带植物引种园园设课题“红树植物老鼠簕提取物质量标准的初步研究”(2023-03)。

摘　　要：为深入研究老鼠簕(Acanthus ilicifolius Linn.)全株的化学成分,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等手段对老鼠簕全株的化学成分进行分离纯化;利用1 H-NMR、13 C-NMR、二维核磁共振等现代波谱技术,并结合理化性质对分离得到的单体化合物进行结构解析和鉴定。共鉴定出12个单体化合物的结构,分别为:(2R)-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮(1)、(2R)-2-羟基-2H-l,4-苯并嗪-3(4H)-酮(2)、(2S)-2-羟基-2H-l,4-苯并嗪-3(4H)-酮(3)、4-羟基-2-苯并唑啉酮(4)、(2R)-7-氯-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮(5)、木犀草素-7-O-β-D-葡萄糖醛酸苷甲酯(6)、芹菜素-7-O-β-D-葡萄糖醛酸苷甲酯(7)、老鼠簕酸(8)、腺嘌呤(9)、(2S)-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮(10)、阿克替苷(11)、异阿克替苷(12)。其中,化合物(6)和(9)为首次从老鼠簕中分离得到。In order to deeply study the chemical components of Acanthus ilicifolius,this study used silica column chromatography,Sephadex LH-20 column chromatography,and other means to separate and purify its chemical components.The separated monomer compounds of Acanthus ilicifolius which were determined by their physicochemical property and modern spectroscopic analysis including 1 H-NMR,13 C-NMR,and 2D NMR.Twelve compounds were isolated and their structures were identified as(2R)-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazine-3(4H)-one(1),(2R)-2-hydroxy-2H-1,4-benzoxazine-3(4H)-one(2),(2S)-2-hydroxy-2H-1,4-benzoxazine-3(4H)-one(3),4-hydroxyl-2-benzoxazolinone(4),(2R)-7-chloro-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazin-3(4H)-one(5),luteolin-7-O-β-D-glucuronopyranoside methyl ester(6),apigenin-7-O-β-D-glucuronopyranoside methyl ester(7),Ilicifolius acids A(8),adenine(9),(2S)-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazin-3(4H)-one(10),acteoside(11),and isoacteoside(12).Compounds 6 and 9 were isolated from Acanthus ilicifolius for the first time.

关 键 词：老鼠簕 化学成分 生物碱 黄酮 苯乙醇苷 

分 类 号：R284.2[医药卫生—中药学]