光倒刺鲃(Spinibarbus hollandi)基因组Survey及线粒体基因组研究  

GENOMIC SURVEY AND MITOCHONDRIAL GENOME STUDY OF SPINIBARBUS HOLLANDI

作　　者：赖洁 叶树政 黄文炜 李斯迅 邓彬华 韩崇 龚剑[2] 桂林[1] 李强[1] LAI Jie;YE Shu-Zheng;HUANG Wen-Wei;LI Si-Xun;DENG Bin-Hua;HAN Chong;GONG Jian;GUI Lin;LI Qiang(School of Life Sciences,Guangzhou University,Guangzhou 510006,China;Key Laboratory for Water Quality and Conservation of the Pearl River Delta,Ministry of Education,School of Environmental Science and Engineering,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]广州大学环境科学与工程学院、珠江三角洲水质安全与保护教育部重点实验室,广东广州510006

出　　处：《海洋与湖沼》2025年第2期423-432,共10页Oceanologia Et Limnologia Sinica

基　　金：广州市科技计划项目,201804010486号;国家自然科学基金资助项目,42077321号;2024年度广东省大学生创新创业项目,S202411078013号。

摘　　要：光倒刺鲃(Spinibarbus hollandi)是我国南部重要经济鱼类,为了解其基因组特征及线粒体基因组结构,首次采用二代高通量测序技术对珠江和赣江水系光倒刺鲃基因组进行Survey分析,并在此基础上提取出两者的线粒体基因组。结果显示珠江和赣江水系光倒刺鲃基因组大小分别为1769.91 Mb和1846.46 Mb,均为简单杂合型基因组。珠江和赣江水系光倒刺鲃线粒体基因组长度分别为16543 bp和16546 bp,除16S rRNA、tRNA-Leu(UUA)和D-loop区外,两水系其他线粒体基因长度一致。光倒刺鲃线粒体蛋白编码基因中出现不完整的密码子T/TA。tRNA中除tRNA-Ser(GCT)外,其他均能折叠形成正常二级结构。利用线粒体基因组序列计算各水系个体间的遗传距离,使用邻接法和贝叶斯法构建系统进化树,结果表明赣江和桐江水系光倒刺鲃亲缘关系最近,台湾水系光倒刺鲃与大陆水系的亲缘关系较远,台湾种群光倒刺鲃可能是有效种。该研究旨在为研究光倒刺鲃群体遗传学和分子系统学提供基础信息,为今后其种质鉴定、保护和开发利用提供科学依据。Spinibarbus hollandi is a commercially important aquaculture species distributed in the southeastern China.To understand its genomic features and mitochondrial genome structure,for the first time,we sequenced the whole genomes of S.hollandi from Zhujiang(Pearl)River(SHZ)and Ganjiang River(SHG)based on Illumina short-read method and subsequently extracted their mitochondrial genomes.Results indicate that the S.hollandi genome is of a simple heterozygous type,with genome sizes of 1769.91 Mb for SHZ and 1846.46 Mb for SHG.The mitochondrial genome lengths are 16543 bp for SHZ and 16546 bp for SHG.Most genes of the two S.hollandi are in the same length except for 16S rRNA,tRNA-Leu(UUA),and D-loop region.Incomplete codons T/TA were observed in the protein-coding genes.With the exception of tRNA-Ser(GCT),all other tRNAs are able to adopt their expected secondary structures.Utilizing mitochondrial genome sequences,genetic distances among various populations were calculated,and the phylogenetic tree was constructed employing both the Neighbor-Joining method and Bayesian method.Results indicate that the genetic relationship is the closest between the S.hollandi from the Tongjiang River and Ganjiang River,while the relationship between the Taiwan population and the mainland populations is the most distant.The Taiwan population of S.hollandi should be considered a distinct species.This study provided key information for understanding the population genetics and molecular systematics of S.hollandi,and a scientific basis for future germplasm identification,conservation,and utilization.

关 键 词：光倒刺鲃 基因组Survey 线粒体基因组 系统发育 

分 类 号：Q789[生物学—分子生物学] S964.5[农业科学—水产养殖] Q953.3[农业科学—水产科学]