瑞香狼毒对小鼠肝脏损伤和糖代谢的影响  

Effect of Stellera chamaejasme L.on Liver Injury and Glucose Metabolism in Mice

作　　者：周于凯 孙富燕 吕玺玮 何晓蝶 李鸿亮 李文鹏 刘道鑫 洪金 高磊 ZHOU Yukai;SUN Fuyan;LYU Xiwei;HE Xiaodie;LI Hongliang;LI Wenpeng;LIU Daoxin;HONG Jin;GAO Lei(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《中国兽医杂志》2025年第4期44-52,共9页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金地区项目(32260907);青海省科技厅自然科学基金青年项目(2022-ZJ-934Q);青海大学青年科研基金项目(2022-QNY-5);青海大学省部共建三江源生态与高原农牧业国家重点实验室自主课题(2022-ZZ-09)。

摘　　要：为了探究有毒植物瑞香狼毒对小鼠肝脏损伤和糖代谢的影响,本试验将小鼠分为对照组、试验组Ⅰ组和试验Ⅱ组,分别饲喂0、5和10 g/(kg·d)的瑞香狼毒,观察小鼠临床症状并检测体重增加量,应用酶联免疫吸附试验(ELISA)检测血清生化指标变化,苏木精-伊红(H.E.)染色检测肝脏组织学变化,实时荧光定量聚合酶链式反应(qPCR)检测糖代谢相关基因腺嘌呤核糖核苷酸活化蛋白激酶-α1(Ampkα1)、信号转导与转录激活因子-3(Stat3)、葡萄糖-6-磷酸酶催化亚基-1(G6pc1)和沉默信息调节因子-1(Sirt1)在小鼠肝脏中的表达变化,蛋白免疫印迹(WB)检测糖代谢蛋白AMPKα1在小鼠肝脏中的表达变化。结果显示,饲喂瑞香狼毒的2个试验组小鼠均出现不同程度的精神沉郁、行动缓慢和被毛杂乱等症状;与对照组相比,试验I组体重增加量在7和28 d时显著降低(P<0.05或P<0.01),试验II组在整个试验过程中均极显著降低(P<0.001);试验II组在14、21和28 d时极显著低于试验I组(P<0.01或P<0.001);与对照组相比,2个试验组血清葡萄糖(GLU)和总蛋白(TP)水平均极显著降低(P<0.001),碱性磷酸酶(ALP)和谷丙转氨酶(ALT)活性均极显著升高(P<0.001),试验II组总胆固醇(TCH)、总胆红素(TBIL)水平和谷草转氨酶(AST)活性均极显著升高(P<0.001);与试验I组相比,试验II组GLU水平极显著降低(P<0.01),TCH、TBIL水平和ALP、ALT、AST活性均极显著升高(P<0.01或P<0.001);2个试验组小鼠肝脏肝细胞索正常结构被破坏、肝细胞坏死并伴有淤血和炎性细胞浸润的病理学变化;与对照组相比,试验I组肝脏Sirt1和G6pc1基因表达量显著下调(P<0.05),试验II组Ampkα1、Stat3和G6pc1基因表达量极显著下调(P<0.01或P<0.001);试验II组Ampkα1、Sirt1、Stat3和G6pc1基因表达量显著或极显著低于试验I组(P<0.05,P<0.01或P<0.001);试验Ⅱ组肝脏AMPKα1蛋白表达量极显著低于对照组和试验I组(P<0.001)。结果表明,瑞�To investigate the effects of the toxic plant Stellera chamaejasme L.on liver injury and glucose metabolism in mice,this study divided the mice into three groups:Negative control group,Experimental feeding I group,and Experimental feeding II group,which were fed 0,5,and 10 g/(kg·d)of Stellera chamaejasme L.,respectively.Clinical symptoms were observed,and body weight gain was measured.Serum biochemical parameters were analyzed using enzyme linked immunosorbent assay(ELISA),and liver histopathological changes were examined through hematoxylin-eosin(H.E.)staining.Quantitative real-time polymerase chain reaction(qPCR)was employed to assess the expression levels of glucose metabolism-related genes,including AMP-activated protein kinaseα1(Ampkα1),signal transducer and activator of transcription 3(Stat3),glucose-6-phosphatase catalytic subunit 1(G6pc1),and sirtuin 1(Sirt1)in mouse liver.Western blot(WB)analysis was conducted to detect AMPKα1 protein expression in liver tissues.The results showed that both experimental groups exhibited varying degrees of depression,slow movement,and unkempt fur.Compared with the Negative control group,body weight gain in Experimental feeding I group was significantly reduced on days 7 and 28(P<0.05 or P<0.01),while in Experimental feeding II group,body weight gain was significantly reduced throughout the experiment(P<0.001).Additionally,body weight gain in Experimental feeding II group was significantly lower than in Experimental feeding I group on days 14,21,and 28(P<0.01 or P<0.001).Compared with the Negative control group,both experimental groups showed significantly reduced glucose(GLU)and total protein(TP)levels(P<0.001)and significantly increased alkaline phosphatase(ALP)and alanine aminotransferase(ALT)activities(P<0.001).In Experimental feeding II group,total cholesterol(TCH),total bilirubin(TBIL)levels,and aspartate aminotransferase(AST)activity were also significantly elevated(P<0.001).Compared with Experimental feeding I group,Experimental feeding II group exhibited signi

关 键 词：瑞香狼毒 小鼠 肝脏 损伤 糖代谢 

分 类 号：S8[农业科学—畜牧兽医] S85