盐生植物中国柽柳响应盐胁迫的代谢组学分析  

Metabolomics Analysis of Halophyte Tamarix chinensis in Response to Salt Stress

作　　者：靳芊芊 吴常伟 殷楠 闫杰 张国君[1] 徐兴兴 JIN Qianqian;WU Changwei;YIN Nan;YAN Jie;ZHANG Guojun;XU Xingxing(College of Horticulture Science and Technology,Hebei Normal University of Science&Technology/Hebei Key Laboratory of Characteristic Horticultural Germplasm Mining and Innovative Utilization/Hebei Provincial University Research and Development Center of Characteristic Horticultural Plant Biological Breeding Application Technology,Qinhuangdao Hebei,066600,China)

机构地区：[1]河北科技师范学院园艺科技学院/河北省特色园艺种质挖掘与创新利用重点实验室/河北省高校特色园艺植物生物育种应用技术研发中心,河北秦皇岛066600

出　　处：《河北科技师范学院学报》2025年第1期30-42,73,共14页Journal of Hebei Normal University of Science & Technology

基　　金：河北省高等学校科学技术研究项目(项目编号:ZD2022091);河北省重点研发计划项目(项目编号:21326346D);河北省高校园艺作物育种应用技术研发中心项目(项目编号:YF201404);河北省级专业学位研究生培养实践基地项目(项目编号:202018);林木遗传育种全国重点实验室开放课题(项目编号:2024KFKT02)。

摘　　要：为了确定盐生植物中国柽柳(Tamarix chinensis Lour.)盐胁迫响应的代谢机制,挖掘差异代谢物及其代谢途径,选择了5种中国柽柳无性系进行盐腺观察和耐盐性生理指标测定,并对耐盐性较强的中国柽柳无性系在400 mmol/L NaCl处理48 h后利用液质联用(LC-MS)技术分析体内代谢物变化情况。结果显示:5种中国柽柳无性系中2号盐腺直径大,盐腺密度也相对较多;2号中国柽柳无性系在400 mmol/L NaCl处理48 h生理指标数值最好;对差异代谢通路进行通路富集分析表明正离子和负离子模式下共富集到膜运输代谢通路,因此膜运输代谢通路参与了中国柽柳响应盐胁迫机制;盐胁迫下中国柽柳中代谢组分析出正负离子共有825个代谢物。ABC转运蛋白、苯丙氨酸代谢、脯氨酸代谢等途径都参与了盐胁迫的调控。盐胁迫能造成中国柽柳无性系中的代谢物发生显著变化。Five Tamarix chinensis asexual lines were selected for salt gland observation and determination of physiological indexes of salinity tolerance,and the changes of Tamarix chinensis asexual lines with stronger salinity tolerance were analyzed with liquid mass spectrometry(LC-MS)after 48 h of 400 mmol/L NaCl treatment to determine the metabolic mechanism of salt stress response in halophytes Tamarix chinensis,and to explore the differential metabolites and their metabolic pathways.The results showed that among five Tamarix chinensis asexual lines,No.2 had larger diameter and higher density of salt glands;No.2 had the best value of physiological indexes after 48 h of 400 mmol/L NaCl treatment;the pathway enrichment analysis of different metabolic pathways showed that the positive and negative ion modes were enriched to the membrane transport metabolic pathway,indicating that the main metabolic pathway of the Tamarix chinensis under salt stress was the membrane transport pathway;825 metabolites of positive and negative ions were analyzed in the metabolome of Tamarix chinensis under salt stress.ABC transporter protein,phenylalanine metabolism,and proline metabolism were involved in the regulation of salt stress,resulting in significant changes in metabolites in Tamarix chinensis asexual lines.

关 键 词：盐生植物 中国柽柳 盐胁迫 非靶向代谢组学分析 

分 类 号：S793.5[农业科学—林木遗传育种]