聚腺嘌呤介导的适配体修饰Au@Pt纳米酶探针检测水中Pb^(2+)  

Poly-adenine Mediated Aptamer Modified Au@Pt Nanozyme Probe for the Determination of Pb^(2+) in Water

作　　者：薛梅[1] 白龙 苏采薇 叶泰[1] XUE Mei;BAI Long;SU Caiwei;YE Tai(Shanghai Engineering Technology Research Center for Rapid Food Detection,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海食品快速检测工程技术研究中心,上海理工大学健康科学与工程学院,上海200093

出　　处：《中国无机分析化学》2025年第5期609-617,共9页Chinese Journal of Inorganic Analytical Chemistry

基　　金：国家自然科学基金面上基金资助项目(32172303);上海市启明星项目(22QA1406500)。

摘　　要：铅(Pb)作为典型的有毒重金属元素,其环境迁移特性已被证实具有显著的生物毒性效应,而传统的Pb^(2+)检测方法往往依赖于复杂的设备和专业的操作人员。因此开发简便、灵敏的Pb^(2+)检测平台对于食品安全监控及毒理学评估十分重要。为了简便、灵敏检测Pb^(2+),采用聚腺嘌呤将适配体锚定在具有核壳结构的金-铂双金属纳米酶表面,构建了用于Pb^(2+)灵敏检测的纳米酶探针(Au@Pt-polyAT30695)。在金纳米颗粒表面引入铂壳层,不仅提高了纳米酶的过氧化物模拟酶活性,同时利用金属铂对腺嘌呤高亲和力,实现了纳米酶表面适配体的可控固定,有效保障了纳米酶探针的识别效能。Pb^(2+)存在下,Au@Pt-polyAT30695表现出更强的过氧化物模拟酶活性,催化Amplex UltraRed (AUR)氧化荧光增强。系统考察了铂壳厚度和聚腺嘌呤数量对Pb^(2+)响应的影响,在最佳检测条件下,溶液的荧光强度增益比与Pb^(2+)的浓度(0.01~300 nmol/L)呈线性相关,检出限为3.47 pmol/L(S/N=3)。此外,将该探针用于黄浦江水样加标回收实验,回收率在93.0%~113%。结果表明,构建的Au@Pt-polyAT30695探针能够简便、灵敏地检测Pb^(2+)且在实际水样中具有潜在的应用价值,能够为环境水体中Pb^(2+)监测提供有效的技术手段。Lead (Pb) is a typical toxic heavy metal element that poses significant biotoxic risks due to its environmental transport properties.Pb-containing contaminants are transported into agro-ecosystems via water bodies,and then through biogeochemical cycles,they move from irrigation water to soil and crops,eventually accumulating in the edible parts of crops.This pollutant ultimately enters the human digestive system through dietary exposure,exhibits cumulative effects in the human body,and causes irreversible damage to the nervous,hematopoietic,and reproductive systems.Consequently,Pb contamination has been classified as one of the priority environmental health risks globally.Traditional Pb^(2+)detection methods often rely on complex equipment and specialized operators,which limits their practical application.Therefore,developing a simple and sensitive Pb^(2+)detection platform is crucial for food safety monitoring and toxicological evaluation.In this study,we constructed a nanoenzyme probe (Au@Pt-polyAT30695) for the sensitive detection of Pb^(2+).Polyadenine was used to anchor the aptamer on the surface of gold-platinum bimetallic nanoparticles with a core-shell structure.The introduction of a platinum shell layer on the gold nanoparticle surface not only enhanced the peroxidase-mimicking activity of the nanoenzyme but also enabled controlled immobilization of the aptamers,leveraging the high affinity of platinum for adenine.This design effectively ensured the recognition efficacy of the nanoenzyme probe.In the presence of Pb^(2+),Au@Pt-polyAT30695 exhibited stronger peroxidase-mimetic activity,catalyzing the oxidative fluorescence enhancement of Amplex UltraRed (AUR).We systematically investigated the effects of platinum shell thickness and polyadenine quantity on Pb^(2+)response.Under optimal assay conditions (800μL H_(2)PtCl_(6) addition,9-nt polyA length,0.15 nmol/L Au@Pt800-9AT30695 concentration,75μmol/L AUR concentration,2 mmol/L H_(2)O_(2) concentration,and 120 min reaction time),the fluorescence intensit

关 键 词：纳米酶 适配体 铅离子 金铂纳米颗粒 探针 

分 类 号：O657.34[理学—分析化学] O657.31[理学—化学] X832[环境科学与工程—环境工程]