烟管菌生物学特性、驯化栽培及抗氧化活性  

Biological characteristics,domestic cultivation and antioxidant activities of Bjerkandera adusta

作　　者：康霞 常光雷 任静 王立安[2] 李肖 李守勉[1] 李国杰 KANG Xia;CHANG Guanglei;REN Jing;WANG Li’an;LI Xiao;LI Shoumian;LI Guojie(Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei/College of Horticulture,Hebei Agricultural University,Baoding 071001,China;College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]河北省蔬菜种质资源创新与利用重点实验室/河北省蔬菜产业协同创新中心/河北农业大学园艺学院,河北保定071001 [2]河北师范大学生命科学学院,河北石家庄050024

出　　处：《河北农业大学学报》2025年第2期26-36,共11页Journal of Hebei Agricultural University

基　　金：河北农业大学引进人才科研专项(YJ201849);国家食用菌产业技术体系小宗食用菌岗位(No.CARS-20);河北省科技支撑计划资助项目(2053731D);河北省科技计划项目重点研发计划(21326315D);河北省食用菌产业创新团队珍稀食用菌岗位(HBCT2023090202);河北省县域生物多样性调查评估项目(2011600012).

摘　　要：烟管菌(Bjerkandera adusta)菌种由采自河北省遵化市的野生子实体组织分离获得,采用单因素和正交试验进行生物学特性研究,并进行驯化栽培,测定野生和栽培子实体醇提物对ABTs+、DPPH及羟自由基的清除能力。结果表明,烟管菌菌丝生长最适碳源、氮源、温度和pH分别为果糖、酵母浸粉、30℃和6。正交试验结果表明,温度对其菌丝生长的影响最大,其次为碳源、氮源和pH。驯化栽培结果表明,菌丝发菌时间为20 d;给予300~500 lx的光照,空气湿度为80%~85%,日间保持室温在25~30℃,夜间温度降低至16~18℃,40~45 d后形成原基;将湿度提高至90%~95%,15~20 d后子实体成熟。野生及栽培子实体的醇提物随着添加量的增加抗氧化活性逐渐增强,当添加量为200μL时,野生和栽培子实体对ABTs+、DPPH及羟自由基的清除率均达到最高,野生子实体的清除率分别为84.10%、60.74%和96.06%,显著高于栽培子实体,其清除率分别为70.54%、35.81%和63.16%,二者对以上自由基的清除率分别相差13.56%、24.93%和32.90%。研究结果可为该菌后续开发利用提供参考。The strain of Bjerkandera adusta was isolated from wild fruiting body tissues collected in Zunhua City,Hebei Province.Biological characteristics were evaluated through single factor and orthogonal experiments.Domesticated fruiting bodies were cultivated from this strain.Scavenging ability of alcohol extracts from wild and cultivated fruiting bodies were determined against ABTs+,DPPH and·OH were carried out.The results showed that optimal carbon and nitrogen source,temperature,as well as pH for mycelial growth were fructose,yeast extract powder,30℃,and pH 6.Orthogonal experiments showed that temperature had the greatest effect on the growth of mycelium.The subordinate factors were carbon source,nitrogen source and pH.Domestication cultivation indicated that mycelia were sackful after 20 days;B.adusta needed 40~45 days to form primordium under the conditions of 300~500 lx of light,80%~85%humidity,25~30℃during the day and 16~18℃at night.Fruiting bodies matured in 15~20 days after humidity was increased to 90%~95%.Antioxidant activities of alcohol extracts of both wild and cultivated fruiting bodies were gradually increased as the additive amount raising.Scavenging rates of ABTs+,DPPH and·OH reached 84.10%,60.74%and 96.06%respectively in wild fruiting bodies when additive amount was 200μL,which were significantily higher than cultivated fruiting bodies.Scavenging rates of cultivated fruiting bodies reached 70.54%,35.81%and 63.16%,respectively.The difference in the scavenging rate of above free radicals between wild and cultivated fruiting bodies were 13.56%,24.93%and 32.90%,respectively.This research provided a reference for further development and utilization of B.adusta.

关 键 词：烟管菌 单因素 药用真菌 正交试验 

分 类 号：S046[农业科学]