灰葡萄孢致病蛋白BcPDR1的功能区域鉴定  

Identification of the functional region of the pathogenic protein BcPDR1 in Botrytis cinerea

作　　者：刘晓颖 魏雅迪 张天雨 曹宏哲 张康 董金皋[1] 藏金萍[1] 邢继红[1] LIU Xiaoying;WEI Yadi;ZHANG Tianyu;CAO Hongzhe;ZHANG Kang;DONG Jingao;ZANG Jinping;XING Jihong(Hebei Key Laboratory of Plant Physiology and Molecular Pathology/Laboratory of Mycotoxins and Plant Molecular Pathology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室,河北保定071000

出　　处：《河北农业大学学报》2025年第2期62-69,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(32072369);河北省自然科学基金(C2022204040);中央引导地方科技发展资金(246Z6506G).

摘　　要：灰葡萄孢(Botrytis cinerea)是一种典型的死体营养型植物病原真菌,能够危害上千种植物并引起灰霉病。本实验室的前期研究发现了1个新的灰葡萄孢致病基因BcPDR1(Pathogenicity Deficient Related 1),明确了该基因正调控病菌的生长发育和致病力。然而,生物信息学分析未发现该基因的同源基因及其保守结构域。为了探明该基因的作用机制,本研究对灰葡萄孢BcPDR1的重要功能区域进行了分析,将BcPDR1蛋白分为3个区域:BcPDR1-1(1~80 aa)、BcPDR1-2(81~121 aa)和BcPDR1-3(122~199 aa)。利用基因互补回复技术,在BcPDR1基因敲除突变体ΔBcpdr1的基础上构建了3个区域的回复突变体。对3个区域回复突变体的表型和致病力进行分析,结果显示,3个区域回复突变体的菌落形态、菌丝形态、生长速率和侵染结构均与突变体ΔBcpdr1基本一致,在番茄果实和烟草叶片上均能产生明显的病斑,其中回复突变体BcPDR1-3的病斑最为显著,表明BcPDR1-3(122~199 aa)是BcPDR1蛋白的重要功能区域。研究结果为阐明灰葡萄孢致病蛋白BcPDR1调控病菌生长发育及致病分子机制以及灰霉病防治靶标药物的研发奠定了基础。Botrytis cinerea is a typical necrotrophic plant pathogenic fungus that can damage thousands of plant species and cause gray mold.Previous studies conducted in our laboratory identified a novel pathogenic gene,BcPDR1(Pathogenicity Deficient Related 1),which positively regulates the growth,development,and pathogenicity of B.cinerea.However,bioinformatics analyses did not reveal any homologous genes or conserved domains associated with BcPDR1.To investigate the mechanism of this gene,we analyzed the important functional regions of BcPDR1 in this study.The BcPDR1 protein was divided into three regions:BcPDR1-1(1~80 amino acids),BcPDR1-2(81~121 amino acids),and BcPDR1-3(122~199 amino acids).Using the BcPDR1 gene knockout mutantΔBcpdr1,we constructed three reverse mutants through a gene complementation recovery technique.We then analyzed the phenotypes and pathogenicity of these three regional reversion mutants.The results showed that the colony morphology,mycelial morphology,growth rate,and infection structures of the three regional reversion mutants were largely consistent with those of theΔBcpdr1 mutant.Notably,significant disease spots were observed on tomato fruits and tobacco leaves,with the lesion produced by the reversion mutant BcPDR1-3 being the most pronounced.This finding suggested that BcPDR1-3(122~199 amino acids)was the crucial functional region of the BcPDR1 protein.These results provide a solid foundation for elucidating the molecular mechanisms by which BcPDR1 regulates the growth and development of pathogens,as well as for the development of targeted drugs for the prevention and treatment of gray mold.

关 键 词：灰葡萄孢 BcPDR1 功能区域 生长发育 致病力 

分 类 号：S432.1[农业科学—植物病理学]