酶响应型荧光探针在耐药菌检测中的应用  

Application of enzyme-responsive fluorescent probes in detecting drug-resistant bacteria

作　　者：管佳赟 张靖怡 王励 于滟扬 王琦[1] 秦玉岭 吴丽 GUAN Jiayun;ZHANG Jingyi;WANG Li;YU Yanyang;WANG Qi;QIN Yuling;WU Li(School of Public Health,Nantong University,Nantong 226019,China;School of Life Sciences,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学公共卫生学院,江苏南通226019 [2]南通大学生命科学学院,江苏南通226019

出　　处：《南通大学学报(自然科学版)》2025年第1期1-9,共9页Journal of Nantong University(Natural Science Edition)

基　　金：国家自然科学基金面上基金项目(32171452,82373456);国家自然科学基金青年科学基金项目(62005132)。

摘　　要：随着临床抗生素的过度使用,耐药菌的种类和耐药的类型不断增多。细菌菌株及时、准确的鉴定对抗生素的合理使用至关重要。用于细菌关键酶类代谢物检测的荧光探针作为一种新型的检测工具,克服了传统检测方法的局限性,在耐药菌检测中展现出极大的潜力和优势。文章以临床常见耐药菌关键酶类代谢物检测荧光探针为主题,总结了近年来酶响应型荧光探针技术在耐药菌检测中的应用及其原理。综述了细菌耐药机制与酶代谢物之间的关系,突出了耐药菌与敏感菌在酶代谢上的差异。随后,根据对各种耐药菌特征酶类代谢物的分析,阐明了耐药菌检测荧光探针的工作原理。此外,文章还进一步探讨了荧光探针可能的优化方向,为更快速、更精准的耐药菌诊治提供新的思路。With the overuse of clinical antibiotics,the types and varieties of drug-resistant bacteria are continually increasing.The timely and accurate identification of bacterial strains is crucial for the prevention and control of drug-resistant bacteria and the development of antibiotics.Fluorescent probes for detecting key bacterial enzyme metabolites,as a novel detection tool,can overcome the limitations of traditional detection methods and show great potential and advantages in detecting drug-resistant bacteria.This paper focuses on fluorescent probes for detecting key enzyme metabolites of clinically common drug-resistant bacteria,summarizing the applications and principles of enzyme-responsive fluorescent probe technology in detecting drug-resistant bacteria in recent years.The study reviews the relationship between bacterial resistance mechanisms and enzyme metabolites,highlighting the differences in enzyme metabolism between resistant and sensitive bacteria.Subsequently,based on the analysis of characteristic enzyme metabolites of various drug-resistant bacteria,the working principle of fluorescent probes for detecting drug-resistant bacteria is elucidated.Additionally,the study explores possible directions for optimizing fluorescent probes,providing new insights for faster and more accurate diagnosis and treatment of drug-resistant bacteria.

关 键 词：耐药菌 细菌代谢物 荧光探针 Β-内酰胺酶 硝基还原酶 

分 类 号：R447[医药卫生—诊断学]