常用铁线莲属药材总黄酮及主要活性黄酮苷元含量研究  

Content Determination of Total Flavonoids and Main Active Flavonoid Aglycones in Common Clematis Medicinal Materials

作　　者：吴浩震 杨俊峰 刘玉宁 尼玛次仁 朱业锦 谢和兵 WU Haozhen;YANG Junfeng;LIU Yuning;NIMA Ciren;ZHU Yejin;XIE Hebing(School of pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Nantong Haimen Yangtze Delta Drug Advanced Research Institute,Nantong 226133,China;Jiangsu Shenhou Pharmaceutical Research Co.,Ltd.,Nantong 226133,China;Tibet Shenhou Pharmaceutical Co.,Ltd.,Shigatse 857000,China;School of Medicine&Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]南通市海门长三角药物高等研究院,江苏南通226133 [3]江苏神猴医药研究有限公司,江苏南通226133 [4]西藏神猴药业有限责任公司,西藏日喀则857000 [5]南京中医药大学医学院·整合医学院,江苏南京210023

出　　处：《中医药学报》2025年第4期38-42,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(82304595);西藏自治区科技厅区域科技协同创新专项(QYXTZX-RKZ2022-07)。

摘　　要：目的:研究常用铁线莲属药材总黄酮及主要活性黄酮苷元含量。方法:以2020版《中国药典》《中华人民共和国卫生部药品标准》收载的东北铁线莲、威灵仙、棉团铁线莲、川木通、唐古特铁线莲、细叶铁线莲6种常用铁线莲属药材以及采集到的威灵仙叶、川木通叶为研究对象,采用紫外分光光度法和高效液相色谱法分别测定总黄酮以及主要活性黄酮苷元槲皮素、山柰酚、木犀草素、芹菜素含量。结果:细叶铁线莲中总黄酮的含量最高,其次分别是威灵仙叶、川木通叶、唐古特铁线莲、东北铁线莲、威灵仙、棉团铁线莲和川木通;威灵仙、东北铁线莲、棉团铁线莲、川木通中不含主要活性黄酮苷元,细叶铁线莲中槲皮素、木犀草素、山奈酚含量最高,威灵仙叶中芹菜素含量最高。结论:常用铁线莲属药材中总黄酮含量以及主要活性黄酮苷元组成差异显著,2020年版《中国药典》收载的东北铁线莲、威灵仙、棉团铁线莲、川木通药材不适宜作为黄酮活性成分来源,本研究可为常用铁线莲属药材的综合利用以及修订完善《中国药典》的铁线莲属药材的质量标准提供参考。Objective:To determine the content of total flavonoids and main active flavonoid aglycones in common Clematis medicinal materials.Methods:Using six commonly used Clematis medicinal materials recorded in the 2020 edition of the Chinese Pharmacopoeia and Drug Standards of the Ministry of Health of the People’s Republic of China(Clematis mandshurica,Clematis chinensis,Clematis hexapetala,Clematis armandii,Clematis tangutica,and Clematis australis),as well as collected Clematis chinensis leaves and Clematis armandii leaves as study objects.Total flavonoid content and the content of main active flavonoid aglycones(quercetin,kaempferol,luteolin,and apigenin)were determined by ultraviolet spectrophotometry and high-performance liquid chromatography(HPLC).Results:The total flavonoid content was highest in Clematis australis,followed by Clematis chinensis leaves,Clematis armandii leaves,Clematis tangutica,Clematis mandshurica,Clematis chinensis,Clematis hexapetala,and Clematis armandii.The main active flavonoid aglycones were absent in Clematis chinensis,Clematis mandshurica,Clematis hexapetala,and Clematis armandii.The content of quercetin,luteolin,and kaempferol was highest in Clematis australis,while the content of apigenin was highest in Clematis chinensis leaves.Conclusion:Significant differences were observed in the total flavonoid content and composition of main active flavonoid aglycones among commonly used Clematis medicinal materials.The Clematis medicinal materials recorded in the 2020 edition of the Chinese Pharmacopoeia(Clematis mandshurica,Clematis chinensis,Clematis hexapetala,and Clematis armandii)are not suitable as sources of flavonoid active ingredients.These findings provide a reference for the comprehensive utilization of Clematis medicinal materials and the revision of Chinese Pharmacopoeia quality standards for Clematis medicinal materials.

关 键 词：铁线莲 总黄酮 紫外分光光度计 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]