病毒样颗粒的制备研究与应用进展  

Advances in the Preparation and Application of Virus-like Particles

作　　者：朱珊 刘志安 李春 胡宇峰 姚新欣 ZHU Shan;LIU Zhian;LI Chun;HU Yufeng;YAO Xinxin(State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases,Gannan Innovation and Translational Medicine Research Institute,Gannan Medical University,Ganzhou 341000,China;The First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,China)

机构地区：[1]赣南医科大学赣南创新与转化医学研究院、重大疾病新药靶发现及新药创制全国重点实验室,赣州341000 [2]赣南医科大学第一附属医院,赣州341000

出　　处：《中国生物工程杂志》2025年第2期68-79,共12页China Biotechnology

基　　金：国家自然科学基金青年科学基金(82300684);江西省科技厅重点研发计划(20223BBG71008);章贡区2022年科技计划项目“科技重大专项”(区科字[2022]23号)资助项目。

摘　　要：病毒样颗粒(virus like particle,VLP)是含有特定病毒的一个或多个结构蛋白的自组装纳米级颗粒,具有强免疫原性,它们在结构和构象上与天然病毒相似,但不含有病毒基因组,这使得VLP成为一种安全且有效的生物学工具。近年来,VLP在疫苗开发、药物递送、基因编辑等领域取得了显著进展。概括了VLP的结构特性,不同VLP表达系统的优缺点,VLP的纯化策略,以及VLP在疫苗、药物递送、基因编辑、肿瘤免疫治疗等方面的应用,以期为VLP技术的研究和应用提供参考,使其在生物医药领域发挥更大作用。Virus-like particles(VLPs)are self-assembled nanoscale particles containing one or more structural proteins of a specific virus,which are highly immunogenic.They resemble natural viruses in structure and conformation,but do not contain viral genomes,making VLPs a safe and effective biological tool.In recent years,VLPs have made remarkable progress in the fields of vaccine development,drug delivery,and gene editing.The article outlines the structural properties of VLPs,the advantages and disadvantages of different VLP expression systems,the purification strategies of VLPs,and the applications of VLPs in vaccines,drug delivery,gene editing,and tumor immunotherapy,with the aim of providing references for the research and application of VLP technology and making it more useful in the biomedical environment.

关 键 词：病毒样颗粒 疫苗 药物递送 基因编辑 

分 类 号：Q819[生物学—生物工程] R392[医药卫生—免疫学]