Experimental study of a novel mouse model of tibial shaft fracture combined with blunt chest trauma  

作　　者：Bing Zhang Tingting Fu Yi Han Gongke Li Xianyao Wan Yong Li 

机构地区：[1]Department of Emergency Intensive Care Medicine,Affiliated Hospital of Yangzhou University,Yangzhou,China [2]Department of Special Care Medicine,Affiliated Hospital of Yangzhou University,Yangzhou,China [3]Department of Clinical Medicine,Medical College of Yangzhou University,Yangzhou,China [4]Department of Critical Care Medicine,Affiliated Hospital of Dalian Medical University,Dalian,China [5]Department of Intensive Care Medicine,Affiliated Hospital of Yangzhou University,Yangzhou,China

出　　处：《Animal Models and Experimental Medicine》2025年第4期696-706,共11页动物模型与实验医学(英文)

基　　金：This study was supported by Development Project of Key Laboratory of Big Data Analysis and Knowledge Service of Science and Technology Innovation Platform of Yangzhou-Yangzhou University Cooperation,Grant/Award Number:YBK202204;Jiangsu Province 333 High-level Talent.Training Project,Grant/Award Number:BRA2020176。

摘　　要：Backgroud:Thoracic Trauma and Limb Fractures Are the Two most Common Injuries in Multiple Trauma.However,there Is Still a Lack of Mouse Models of Trauma Combining Tibial Shaft Fracture(TSF)and Thoracic Trauma.In this Study,we Attempted to Develop a Novel Mouse Model of TSF Combined with Blunt Chest Trauma(BCT).Methods:A total of 84 C57BL/6J male mice were used as the multiple trauma model.BCT was induced by hitting the chests of mice with heavy objects,and TSF was in-duced by hitting the tibia of mice with heavy objects after intramedullary fixation.Serum specimens of mice were received by cardiac puncture at defined time points of 0,6,12,24,48,and 72 h.Results:Body weight and body temperature tended to decrease within 24 h after mul-tiple trauma.Hemoglobin analyses revealed a decrease during the first 24 h after mul-tiple trauma.Some animals died by cardiac puncture immediately after chest trauma.These animals exhibited the most severe pulmonary contusion and hemorrhage.The level of lung damage varied in diverse mice but was apparent in all animals.Classic he-matoxylin and eosin(H&E)-stained paraffin pulmonary sections of mice with multiple trauma displayed hemorrhage and an immunoinflammatory reaction.Bronchoalveolar lavage fluid(BALF)and serum samples of mice with multiple trauma showed an upreg-ulation of interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-1α(TNF-1α)compared with the control group.Microimaging confirmed the presence of a tibia fracture and pulmonary contusion.Conclusions:The novel mouse multiple trauma model established in this study is a common trauma model that shows similar pathological mechanisms and imaging characteristics in patients with multiple injuries.This study is useful for determining whether blockade or intervention of the cytokine response is beneficial for the treat-ment of patients with multiple trauma.Further research is needed in the future.

关 键 词：animal model CYTOKINES MICROIMAGING multiple trauma pulmonary contusion 

分 类 号：R683[医药卫生—骨科学]