藜蒿ISSR分子标记遗传多样性评价  

Genetic Diversity Evaluation of ISSR Molecular Markers in Artemisia selengensis

作　　者：陈美珠 董笑笑 夏忙 王宇航 曾长立[1] CHEN Meizhu;DONG Xiaoxiao;XIA Mang;WANG Yuhang;ZENG Changli(School of Life Sciences,Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学生命科学学院,湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心,湖北武汉430056

出　　处：《江汉大学学报(自然科学版)》2025年第2期26-33,共8页Journal of Jianghan University:Natural Science Edition

基　　金：湖北省重点研发计划项目(2022BBA0064);武汉市产学研项目(CXY202203);江汉大学一流学科建设重大科研专项(2023XKZ026)。

摘　　要：藜蒿是多年生草本植物,分布广泛,遗传背景复杂,系统分类模糊。为了解不同种群藜蒿亲缘关系和遗传多样性,利用ISSR分子标记技术对我国9个省份不同地区的117份藜蒿种质资源进行全面分析。通过9条ISSR引物检测到87个多态性位点数,多态性百分比为98.86%。不同群体之间的Nei′s遗传多样性指数(H)平均值为0.0996,Shannon遗传多样性指数平均值为0.1522。利用UPGMA聚类分析117份藜蒿种质,遗传系数为0.35时分为3个主要类群。其中,编号为41~44、110的5个样本归为第一类;其余的大多数样本归为第二类;编号47单独归为第三类。表明不同藜蒿种质资源亲缘关系密切,遗传相似指数高。Artemisia selengensis is a perennial herb with a wide distribution,complex genetic background,and ambiguous systematic classification.The phylogenetic relationships of different populations of Artemisia selengensis should be understood to lay the foundation for genetic improvement.This study used ISSR molecular marker technology to comprehensively analyze 117 germplasm resources of Artemisia selengensis collected from different regions in 9 provinces of China.Using 9 ISSR primers,87 polymorphic loci were detected,with a polymorphism percentage of 98.86%.The average Nei′s genetic diversity index(H)between different populations was 0.0996,and the average Shannon genetic diversity index was 0.1522.UPGMA cluster analysis grouped the 117 Artemisia selengensis samples into three main clusters at a genetic coefficient of 0.35.Among them,most samples belonged to the second cluster,five samples numbered 41-44 and 110 were classified into the first cluster,and the sample numbered 47 formed the third cluster alone.The different germplasm resources of Artemisia selengensis have close phylogenetic relationships and high genetic similarity indices.

关 键 词：藜蒿 ISSR 分子标记 遗传多样性 聚类分析 

分 类 号：Q943.2[生物学—植物学] S567.23[农业科学—中草药栽培]