僵蚕形成过程中蛋白表达谱分析及共表达网络构建  

Analysis of protein expression proffles and construction of co-expression networks during the formation of Bombyx batryticatus

作　　者：张梦姣 李雪敏[1] 段婷引 李玟 赵丹 王巍[1,2] 邓放 ZHANG Mengjiao;LI Xuemin;DUAN Tingyin;LI Wen;ZHAO Dan;WANG Wei;DENG Fang(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Chengdu Food Inspection Institute,Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation,611133,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,成都611137 [2]成都市食品检验研究院国家市场监管重点实验室(营养与健康化学计量及应用),成都611133

出　　处：《应用与环境生物学报》2025年第2期271-281,共11页Chinese Journal of Applied and Environmental Biology

基　　金：四川省科技厅重点研发计划项目(22ZDYF0186);成都中医药大学杏林学者项目(XCZX2022008)资助。

摘　　要：为揭示僵蚕形成过程中蛋白表达谱的变化,深入探究僵蚕形成机制,选定被白僵菌感染的6个不同时间段的家蚕样品为实验材料,采用非标记定量蛋白质组学方法结合纳升反相液相色谱-高分辨质谱技术对感染过程的蛋白质组进行比较,以差异倍数大于2,P <0.05为标准筛选差异表达蛋白(DAPs).共筛选出846个DAPs,其中有7个为共有DAPs.对鉴定蛋白质数据进行相关性分析发现,在感染第5天前后组织样本形成了明显的聚类差异;并且随着感染时间的延长,差异表达蛋白的数量表现为先增加后减少,上调的DAPs与下调的DAPs比率持续增长. GO功能和KEGG通路富集显示差异蛋白主要富集在胞外和核糖体结构中,提供了与僵蚕形成有关的代谢途径和通路相关的蛋白质信息.加权基因共表达网络分析(WGCNA)将构建的僵蚕蛋白共表达网络共聚类分成5个模块,通过模块与表型性状的关联分析,不同时间段的核心蛋白均具有核糖体蛋白.本研究表明僵蚕形成过程中蛋白质存在显著差异以及僵化阶段可能是僵蚕形成的重要步骤,为理解病原真菌与宿主昆虫的相互作用和僵蚕形成的分子机制提供了参考.(图7表2参27)The aim of this study was to reveal changes in protein expression proffles during the formation of stippling silkworms to gain deeper insights into the mechanism of Bombyx batryticatus.In this study,six groups of B.mori samples infected with Beauveria bassiana at different time periods were selected as experimental materials.The proteome of the entire infection process was compared using a label-free quantitative proteomics method combined with nano Reverse Liquid Chromatography-High Resolution Mass Spectrometry.Differentially expressed proteins(DAPs)were screened by the criterion of multiplicity of difference greater than 2 and P<0.05.A total of 846 DAPs were screened,of which 8 were shared DAPs.Correlation analysis of the identiffed protein data revealed significant clustering differences between samples collected before and after day 5 of infection.The host larval proteins were gradually converted to fungal proteins upon prolongation of infection.The number of DAPs initially increased and then decreased over time,and the ratio of upregulated and downregulated DAPs continued to grow.GO function and KEGG pathway enrichment analysis showed that DAPs were primarily enriched in extracellular and ribosomal structures,providing insights into proteins related to metabolic pathways and those associated with the formation of B.batryticatus.WGCNA divided the constructed protein co-expression network into ffve modules,and the core proteins at different time periods were analyzed by associating the modules with phenotypic traits,with ribosomal proteins as hub proteins at different time periods.This study reveals significant protein profile changes during B.batryticatus formation,highlighting the sclerotization stage as potentially critical.It offers insights into the molecular mechanisms of fungal-insect interactions and B.batryticatus development.

关 键 词：僵蚕 差异表达蛋白 高分辨质谱 生物信息学分析 WGCNA 

分 类 号：S881.2[农业科学—特种经济动物饲养] S885.1[农业科学—畜牧兽医]