面向细胞液-液相分离的医工检测方法研究进展  

Research Progress of Liquid-liquid Phase Separation and Detection Methods in Cells

作　　者：赵尉杰 朱程君[2] 梁晋铭 刘凯[1] 张瑛琪 姚佳烽 ZHAO Weijie;ZHU Chengjun;LIANG Jinming;LIU Kai;ZHANG Yingqi;YAO Jiafeng(College of Electrical and Mechanical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China;Department of Oncology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Emergency Department of the Third Hospital of Hebei Medical University,Shijiazhuang 050052,China)

机构地区：[1]南京航空航天大学机电学院,江苏南京210016 [2]南京医科大学第一附属医院肿瘤科,江苏南京210029 [3]河北医科大学第三医院急诊科,河北石家庄050052

出　　处：《机械制造与自动化》2025年第2期1-13,共13页Machine Building & Automation

基　　金：国家自然科学基金面上项目(62471225);2023年度河北省中央引导地方科技发展资金项目(236Z7731G)。

摘　　要：液-液相分离(LLPS)在细胞生命活动中起着重要的作用,LLPS的稳态失衡与多种疾病的发展相关。因此,探究LLPS的原理并对其进行检测十分重要。概括LLPS近年来的发展情况,说明细胞中LLPS现象与人体健康的紧密联系。对LLPS的产生原理从热力学与细胞内部驱动力两方面进行分析与总结,指出细胞中LLPS现象与自由能、化学势以及多种物质的驱动作用有关。重点介绍几种LLPS的检测方法,主要讨论显微镜与荧光标记在LLPS检测中的应用,提出将电阻抗成像(EIT)技术用于LLPS检测,满足实时、快速、免标记的检测需求,同时对LLPS未来的发展情况进行了展望。Since liquid-liquid phase separation(LLPS)plays an important role in cell life activities,and its abnormal activities may lead to the production of diseases,it is very important to explore the principle of LLPS and detect it.This paper summerizes the recent development of LLPS,and illustrates the close relationship between LLPS phenomenon in cells and human health.The generation principle of LLPS is analyzed and summarized from two aspects of thermodynamics and internal driving force of cells,which indicates that LLPS phenomenon in cells is related to free energy,chemical potential and driving effect of various substances in the body.Several LLPS detection methods are introduced,and the application of microscope and fluorescent labeling in LLPS detection is mainly discussed.The electrical impedance tomography(EIT)technology applied to the LLPS detection is proposed to meet the requirements of real-time,fast and free marker detection,and the LLPS future development is prospected as well.

关 键 词：液-液相分离 形成原理 检测方法 电阻抗成像技术 发展应用 

分 类 号：TP274.5[自动化与计算机技术—检测技术与自动化装置]