Influence of Interspecies Interactions on Bacterial Community Assembly in the Active and Permafrost Layers on the Qinghai-Tibet Plateau  

作　　者：Hongfei Chi Xiufeng Yin Xuefeng Zhang Jingyi Zhu Lin Zhao Yongcui Deng Pengfei Liu Yongqin Liu 

机构地区：[1]College of Ecology,Lanzhou University,Lanzhou 730000,China [2]Center for the Pan-Third Pole Environment,Lanzhou University,Lanzhou 730000,China [3]State Key Laboratory of Cryospheric Science,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Science,Lanzhou 730000,China [4]State Key Laboratory of Tibetan Plateau Earth System,Resources and Environment(TPESRE),Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China [5]College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China [6]School of Geographical Sciences,Nanjing University of Information Science and Technology,Nanjing 210044,China [7]School of Geography,Nanjing Normal University,Nanjing 210023,China [8]Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application,Nanjing 210023,China

出　　处：《Journal of Earth Science》2025年第2期395-407,共13页地球科学学刊(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China for Excellent Young Scientists Fund Program(No.42222105);the National Natural Science Foundation of China General Program(No.42171144);the Assessment of Ecosystem Carbon Stock and Turnover Patterns in Qinghai Province(No.2021-SFA7-1-1);the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(No.2021QZKK0100)。

摘　　要：Bacterial communities play a crucial role in permafrost biogeochemical cycling and ecosystem function maintenance.Bacterial interaction is one of the main factors in shaping soil bacterial communities.However,how would bacterial interaction influence the bacterial communities in permafrost of the Qinghai-Tibet Plateau(QTP)remains largely unknown.Here we collected paired soil samples from both the active and permafrost layers of two typical QTP permafrost regions in October 2020 for Tuotuohe River(TTH)and May 2022 for Aerjin(ARJ),and investigated the bacterial communities and the role of interactions in structuring the bacterial community and its assembly process through amplicon sequencing of the 16S rRNA gene.Our study revealed distinct bacterial communities,with significant differences in the relative abundances of Proteobacteria(P<0.05),Acidobacteriota(P<0.001),Bacteroidota(P<0.05),and Planctomycetota(P<0.001)between the active layer and the permafrost layer.More importantly,we found that interspecies interactions,including both positive and negative associations,were strongly correlated with bacterial alpha-diversity and played a significant role in community variation and assembly process.Our findings also showed that the community assembly in both the active and permafrost layers was primarily driven by homogeneous selection of deterministic processes,with interspecies interactions accounting for more than 58%and 63%of all assembly mechanisms,respectively.This is the first study to quantify the contribution of bacterial interactions in shaping the bacterial community and its assembly process in permafrost of QTP,highlighting the importance of considering interspecies interactions in future modeling efforts.Our work also emphasizes the necessity of including interspecies interactions in microbial process projections to reduce uncertainty.

关 键 词：PERMAFROST the Qinghai-Tibet Plateau bacterial community Interspecies interaction community assembly process biogeology 

分 类 号：Q938[生物学—微生物学]