NaCl胁迫下鹰嘴紫云英根系基因差异表达及相关通路分析  

Differentially expressed genes and related pathways in root systems of Astragalus cicer under NaCl stress

作　　者：马婷 陈奋奇 王勇 哈雪 李亚君 马晖玲[1] MA Ting;CHEN Fen-qi;WANG Yong;HA Xue;LI Ya-jun;MA Hui-ling(Pratacultural College,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem of Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2025年第4期104-123,共20页Acta Prataculturae Sinica

基　　金：苜蓿育种项目(2022ZD0401102)资助。

摘　　要：土壤盐渍化严重影响植物的生长和产量,限制农业生态经济的发展。鹰嘴紫云英作为优良生态草种、高效绿肥作物和优质蛋白饲草,探究其对NaCl胁迫适应性的分子机制,对提高鹰嘴紫云英的耐盐性,促进其在盐渍土中的栽培具有重要意义。本研究对NaCl胁迫下不同时间点(0、12、48和72 h)的鹰嘴紫云英根系进行转录组学分析,以探究鹰嘴紫云英对NaCl胁迫的响应机制。结果表明,与0 h相比,NaCl胁迫处理12、48和72 h后鹰嘴紫云英根系中分别有46051、45653和42869个差异表达基因,包括上调基因8027、10053和11042个,下调基因38024、35600和31827个。GO富集和KEGG富集显示,鹰嘴紫云英根系主要通过氧化还原酶活性、类黄酮生物合成和苯丙素生物合成等通路来响应NaCl胁迫。通过趋势分析,发现苯丙素生物合成和类黄酮生物合成通路的差异基因在胁迫处理48 h上调表达或恢复到初始量。C2H2、C3H、NAC、MYB、WRKY和bZIP等转录因子在胁迫处理不同时间点下均有表达,可能与鹰嘴紫云英的耐盐性密切相关。本研究结果为进一步探究鹰嘴紫云英耐盐机制提供了基础数据,也为后续提高鹰嘴紫云英的耐盐性提供了理论支撑。Soil salinization seriously affects the growth and yield of plants,and limits the development of agricultural and ecological economies.Astragalus cicer is a versatile ecological grass species,an efficient green manure crop,and a superior protein forage.Exploring the molecular mechanism of its adaptability to NaCl stress is of great significance for enhancing its salt tolerance and advancing its cultivation in saline soil.To investigate its responses to NaCl stress,transcriptomic analyses were performed on the roots of A.cicer at different time points(0,12,48,and 72 hours)during an NaCl treatment.The results showed that,compared with 0 hour,after 12,48,and 72 hours of NaCl stress,there were,respectively,46051,45653,and 42869 differentially expressed genes(DEGs)in the roots of A.cicer,including 8027,10053,and 11042 up-regulated DEGs and 38024,35600,and 31827 down-regulated DEGs,respectively,at the three time points.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the pathways responding to NaCl stress in the roots of A.cicer were oxidoreductase activity,flavonoid biosynthesis,and phenylpropanoid biosynthesis.A trend analysis showed that the DEGs in phenylpropanoid biosynthesis and flavonoid biosynthesis pathway were still up-regulated or had returned to their baseline levels at 48 hours of NaCl treatment.Genes encoding C2H2,C3H,NAC,MYB,WRKY,and bZIP transcription factors were expressed at different time points during the NaCl treatment,suggesting that these transcription factors are closely related to salt tolerance in A.cicer.The results of this study provide basic data for further exploration of the mechanism of salt tolerance of A.cicer,and also provide theoretical support for breeding to improve salt tolerance.

关 键 词：鹰嘴紫云英 根系 转录组 NACL胁迫 差异表达基因 代谢通路 

分 类 号：S54[农业科学—作物学]