基于重测序紫花苜蓿高蛋白、高产关联InDel分子标记开发  

Development of high-protein and high-yield associated InDel molecular markers based on re-sequencing in alfalfa

作　　者：冯雅琪 陈嘉慧 张静妮 隋超 陈基伟 刘志鹏[1] 周强[1] 刘文献[1] FENG Ya-qi;CHEN Jia-hui;ZHANG Jing-ni;SUI Chao;CHEN Ji-wei;LIU Zhi-peng;ZHOU Qiang;LIU Wen-xian(State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,Engineering Research Center of Grassland Industry,Ministry of Education,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Clover(Beijing)Eco-Tech Co.Ltd,Beijing 100020,China)

机构地区：[1]兰州大学草种创新与草地农业生态系统全国重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业教育部工程研究中心,兰州大学草地农业科技学院,甘肃兰州730020 [2]克劳沃(北京)生态科技有限公司,北京100020

出　　处：《草业学报》2025年第4期137-149,共13页Acta Prataculturae Sinica

基　　金：农业生物育种国家科技重大专项,国家自然科学基金项目(32441032,32271748);财政部和农业农村部:国家现代农业产业技术体系资助。

摘　　要：随着高通量测序技术的迅猛发展,测序成本大幅降低,测序速度和数据质量均得到了显著提升,极大地推动了紫花苜蓿基因组的深入研究,进而也为紫花苜蓿不同性状关联的分子标记开发提供了重要基础信息。插入/缺失(insertion/deletion,InDel)多态性标记因其分布广泛、密度高、遗传稳定以及重复性强等优点而受到关注,然而目前其在紫花苜蓿中的研究及应用仍十分有限。本研究基于国内外80份紫花苜蓿材料的基因组重测序数据,通过与紫花苜蓿“中苜1号”参考基因组序列比对挖掘InDel变异位点,开发与高蛋白和高产性状相关的InDel标记。结果表明,在全基因组范围内设计的40个标记中,经聚合酶链式反应(polymerase chain reaction,PCR)验证后,成功开发出29个多态性标记,多态性比率达到72.5%。其中,已开发的InDel_25和InDel_39标记可分别用于高产和优质紫花苜蓿材料的鉴定。本研究开发的InDel标记可结合传统育种方法,有望加速高产、优质紫花苜蓿新品种的培育。With the rapid development of high-throughput sequencing technology,the sequencing cost has been greatly reduced,and the sequencing speed and data quality have been significantly improved.These developments greatly promote the in-depth study of the alfalfa(Medicago sativa)genome,and thus provide important basic information for the development of molecular markers for the association of different traits in alfalfa.Insertion/deletion(InDel)polymorphic markers have attracted great attention because of their wide distribution,high density,genetic stability,and high reproducibility,but research about them and their application in alfalfa are still very limited.In this study,based on genome resequencing data of 80 alfalfa germplasm lines sourced from China and abroad,we explored the InDel locus by comparing with the reference genome sequence of alfalfa“Zhongmu No.1”,and identified InDel markers related to high-protein and high-yield traits.It was found that of 40 markers identified on a genome-wide scale,29 polymorphic markers were successfully categorised with a polymorphism ratio of 72.5%after validation by polymerase chain reaction(PCR).Notably,markers designated InDel_25 and InDel_39 are particularly useful for the identification of high-yield and high-quality alfalfa germplasm lines,respectively.It is anticipated that the InDel markers identified in this research will be applied in traditional breeding programs,thereby accelerating the development of new alfalfa varieties with enhanced yield and quality.

关 键 词：紫花苜蓿 InDel分子标记 产量 品质 

分 类 号：S541[农业科学—作物学]