小鼠乳腺上皮细胞热应激模型的建立  

Establishment of A Heat Stress Model for Mouse Mammary Epithelial Cells

作　　者：冉晓威 RAN Xiaowei(Xizang Veterinary Biological Products Factory,Lhasa Xizang 850009,China)

机构地区：[1]西藏自治区兽医生物药品制造厂,西藏拉萨850009

出　　处：《西藏农业科技》2025年第1期67-71,共5页Tibet Journal of Agricultural Sciences

摘　　要：为构建乳腺上皮细胞系(HCⅡ)的热应激模型,将HCⅡ细胞分为常温组(Con组)、热应激组(HS组)和热应激缓和组(HS-组),用CCK-8法测定细胞活性,微板法检测细胞乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性和活性氧(ROS)、一氧化氮(NO)、丙二醛(MDA)含量,流式细胞仪测定细胞凋亡比例和细胞周期。结果发现:与Con组相比,HS组和HS-组细胞活力显著下降,LDH活性及细胞内ROS、MDA、NO含量显著增加,SOD、GSH、T-AOC和CAT活性显著下降,细胞凋亡显著上升,细胞周期发生阻滞。与HS组相比,HS-组MDA、NO含量显著增加,SOD和GSH活性显著下降,细胞凋亡上升。综上得出,42℃热应激处理小鼠乳腺上皮细胞3h,而后37℃缓和6h,可构建小鼠乳腺上皮细胞的热应激模型。To construct a heat stress model for HC cells,HCI cells were divided into normothermic groups(Con),heat stress groups(HS),and heat stress-alleviation groups(HS-),and cellular activity was measured by the CCK-8 method,Cellular lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH)activity,total antioxidant capacity(T-AOC),catalase(CAT),reactive oxygen species(ROS),nitric oxide(NO),and malondialdehyde(MDA)contents were measured by the microplate method.The apoptosis and cell cycle were determined by flow cytometry.The results showed that compared with the Con group,in the HS and HS-groups,cell viability was significantly decreased,LDH activity and intracellular ROS,MDA,and NO contents were significantly increased,SOD,GSH,T-AOC,and CAT activities were significantly decreased,apoptosis was significantly increased,and the cell cycle was blocked.Compared to the HS group,the HS-group showed a significant increase in cellular MDA and NO content,a significant decrease in SOD and GSH activity,and a rise in apoptosis.In conclusion,subjecting mouse mammary epithelial cells to heat stress at 42 C for 3 hours,followed by recovery at 37℃for 6 hours,can establish a mouse mammary epithelial cell heat stress model.

关 键 词：乳腺上皮细胞 细胞活力 抗氧化性能 细胞凋亡 

分 类 号：S852[农业科学—基础兽医学]