Species Demarcation and Comparative Analysis of Amino Acid and Fatty Acid in Four Scylla Species:Unveiling the Nutritional Profiles  

作　　者：NIDA Imtiaz JIN Runze LI Yongliang WU Qingyang MU Changkao WANG Chunlin LI Ronghua 

机构地区：[1]School of Marine Science,Ningbo University,Ningbo 315211,China [2]Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China

出　　处：《Journal of Ocean University of China》2025年第2期483-494,共12页中国海洋大学学报(英文版)

基　　金：funded by the Key Scientific and Technological Grant of Zhejiang(No.2021C02069-6);the Earmarked Fund(No.CARS-48);K.C.Wong Magana Fund in Ningbo University。

摘　　要：This study examined four species of Scylla genus(S.paramamosain,S.olivacea,S.serrata,and S.tranquebarica)morphologically and molecularly.Morphologically,distinct frontal lobe spine shapes and colorations were observed.Using HRM genotyping with CSN9 primers,we identified clear species-specific melting curve distinctions.The Tm values of S.serrata,S.paramamosain,S.tranquebarica and S.olivacea are 79.55℃,80.12℃,78.12℃ and 82.04℃,respectively.Sequencing data validated these results,revealing 28 SNPs and 9 haplotypes.Amino acid analysis showed arginine dominance in S.paramamosain,which was 40.64 mg g^(-1),and a TFAA range of 135.70-149.65 mg g^(-1).A total of 26 fatty acids were identified,while S.paramamosain showed the highest concentration(P<0.05).EPA was a major component(16.17%-23.26%),while S.paramamosain exhibited the highest levels.Principal component analysis and cluster analysis highlighted distinct species groupings based on amino acid and fatty acid profiles.These findings provide comprehensive insights into Scylla species'morphological,molecular,and biochemical diversities.

关 键 词：free amino acids fatty acids HRM morphology SNPS Scylla species 

分 类 号：S917.4[农业科学—水产科学]