Development of a Mixotrophic Strategy for Efficient Cultivation of a Coccolithophore,Chrysotila roscoffensis  

作　　者：ZHANG Luyao HE Zhichao SUN Yan LI Jian CHENG Pengfei AGATHOS Nicolas Spiros ZHOU Chengxu ZHANG Lin HAN Jichang 

机构地区：[1]College of Food Science and Engineering,Ningbo University,Ningbo 315832,China [2]School of Biological and Chemical Engineering,Panzhihua University,Panzhihua 617000,China [3]Earth&Life Institute,Bioengineering Laboratory,Catholic University of Louvain,Louvain-La-Neuve 1348,Belgium [4]Key Laboratory of Applied Marine Biotechnology,Ministry of Education of China,Ningbo University,Ningbo 315832,China

出　　处：《Journal of Ocean University of China》2025年第2期495-505,共11页中国海洋大学学报(英文版)

基　　金：supported by the Ningbo Science and Technology Project(No.2024J176);the Key Program of Science and Technology Innovation in Ningbo(No.2023 Z118);the Zhejiang Provincial Natural Science Foundation of China(No.LY22C190001)。

摘　　要：Coccolithophores have unique ability of dual carbon fixation(photosynthesis and biocalcification)and can accumulate bioactive compounds effectively,thereby showing huge potential for CO_(2) bioremediation and simultaneous production of valueadded substances.However,biotechnological exploration of these microalgae is still very limited.In order to develop efficient cultivation strategies of these organisms,we tested the abilities of Chrysotila roscoffensis,a coccolithophore,to grow under mixotrophic and heterotrophic cultivation conditions.It was confirmed that only glycerol stimulated the cell growth of this microalga in the presence of light.To study the physiological significance of mixotrophy,we conducted cell culture experiments both autotrophically and mixotrophically,the only difference of which was optimal amount of glycerol was added under mixotrophic conditions.The results showed that the addition of glycerol substantially enhanced the lipids and monounsaturated fatty acids accumulation of C.roscoffensis,but imposed slight influences on the photosynthetic capacity,pigments content,polyunsaturated fatty acids content,and biocalcification.We concluded that mixotrophy might be an appropriate strategy for exploration of this species for production of high value molecules.

关 键 词：COCCOLITHOPHORES Chrysotila roscoffensis PHOTOAUTOTROPHY MIXOTROPHY GLYCEROL 

分 类 号：Q945.11[生物学—植物学] Q945.3