山合欢和银合欢根系细菌群落特征  

Characteristics of root bacterial community of native Albizia kalkora and exotic Leucaena leucocephala

作　　者：王雪梅 闫帮国[2] 王蓥燕 黄天志[1] 王立东 白如霞 WANG Xuemei;YAN Bangguo;WANG Yingyan;HUANG Tianzhi;WANG Lidong;BAI Ruxia(School of Resource and Environmental Engineering,Mianyang Normal University,Mianyang 621000,China;Institute of Tropical Eco-Agriculture,Yunnan Academy of Agricultural Sciences,Yuanmou 651300,China;Mianyang Agricultural Products Quality Safety Inspection and Testing Center,Mianyang 621000,China)

机构地区：[1]绵阳师范学院资源环境工程学院,绵阳621000 [2]云南省农业科学院热区生态农业研究所,元谋651300 [3]绵阳市农畜产品质量安全检验检测中心,绵阳621000

出　　处：《生态学报》2025年第7期3279-3292,共14页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(32101363)。

摘　　要：根系微生物来源于土壤微生物,其对植物的生长发育、适应性与入侵性都具有重要的影响,然而对本地豆科和外来豆科的根系内细菌群落特征的认识并不清楚。以干热河谷本地种山合欢和外来种银合欢为研究对象,通过室内控制培养方法,结合高通量测序分析技术,研究山合欢和银合欢在生长过程中根系内细菌群落特征。结果表明:(1)在整个培养期间银合欢的株高和枯叶生物量显著高于山合欢,银合欢的叶片氮磷比、根瘤数量和根瘤重量在培养80天后也显著高于山合欢。(2)山合欢和银合欢根系内细菌的群落组成存在显著差异。在门水平上,山合欢和银合欢的优势菌门均为变形菌门,山合欢的厚壁菌门和髌骨细菌门显著高于银合欢,而拟杆菌门和放线菌门显著低于银合欢;在属水平上,银合欢的中慢生根瘤菌属、Pseudolabrys属、链霉菌属显著高于山合欢,但其慢生根瘤菌属、类芽胞杆菌属、新鞘脂菌属、柯克斯体属、Saccharimonadaceae属显著低于山合欢。(3)植物、时间以及两者的交互作用对根系细菌群落的α多样性指数均无显著的影响,山合欢和银合欢根系内细菌的α多样性无显著差异。(4)代谢是根内细菌群落最主要的功能,在所有处理中约占80%,在培养80天后,细胞生长和死亡、膜运输、碳水化合物代谢、萜类和酮类化合物代谢4个子功能在山合欢和银合欢根系中存在显著差异。综上,山合欢和银合欢的生长特性和根系内细菌群落特征存在显著差异,在未来的植被恢复过程中应考虑这种差异性可能带来的影响。Root microbiota,originating from soil microorganisms,significantly impacts plant growth,development,adaptability,and invasiveness.However,the characteristics of root endophytic bacterial communities in native and exotic legumes remain poorly understood.In this study,combing with high⁃throughput sequencing technology alongside controlled indoor cultivation methods,we examined the root endophytic bacterial community characteristics of native Albizia kalkora and exotic Leucaena leucocephala in a dry,hot valley.The results showed that:(1)Throughout cultivating period,L.leucocephala exhibited significantly greater plant height and litter biomass compared to A.kalkora,and the leaf N∶P ratio,nodule number and nodule weight of L.leucocephala were also significantly higher than those of A.kalkora after 80 days.(2)There were significant differences in the composition of root bacterial community between A.kalkora and L.leucocephala.At the phylum level,the most dominant phylum was Proteobacteria,and the relative abundance of Firmicutes and Patescibacteria in the roots of A.kalkora was significantly higher than that of L.leucocephala,while the relative abundance of Bacteroidetes and Actinobacteria was significantly lower than that of L.leucocephala.At the genus level,L.leucocephala′s roots had a significantly higher relative abundance of Mesorhizobium,Pseudolabrys,and Streptomyces compared to A.kalkora,yet significantly lower relative abundances of Bradyrhizobium,Paenibacillus,Novosphingobium,Aquicella,and Saccharimonadaceae.(3)Plant,culture time and their interaction had no significant effects on theαdiversity indices of root bacterial communities,and the indices of Chao 1,Pielou e,Shannon and Simpson of root bacteria between A.kalkora and L.leucocephala were not significant different.(4)Metabolism was the most important function within the root bacterial community,constituting approximately 80%of the relative abundance.After 80 days,significant differences in cell growth and death,membrane transport,carbohydrate meta

关 键 词：植物入侵 山合欢 银合欢 根系细菌 群落结构 

分 类 号：Q948[生物学—植物学]