苹果β-微管蛋白基因家族鉴定及表达特性分析  

Identification and expression characterization of appleβ-tubulin gene family in apple

作　　者：黄慧玲 王延翔 王悦 宁芹芹 李明[1,3] 郝鹏博 丛柳 白团辉 史江莉[1,3] 焦健 王苗苗[1,3] 刘昱 赵玉洁 万然 申亚文[1,3] 张恒涛 张坤玺[1,3] 郑先波 HUANG Huiling;WANG Yanxiang;WANG Yue;NING Qinqin;LI Ming;HAO Pengbo;CONG Liu;BAI Tuanhui;SHI Jiangli;JIAO Jian;WANG Miaomiao;LIU Yu;ZHAO Yujie;WAN Ran;SHEN Yawen;ZHANG Hengtao;ZHANG Kunxi;ZHENG Xianbo(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,Henan,China;Zhengzhou Fruit Research Institute,Chinese Academy of Agriculture Sciences,Zhengzhou 450009,Henan,China;Research Center for Apple Engineering and Technology of Germplasm Innovation and Utilisation of Henan Province,Zhengzhou 450046,Henan,China)

机构地区：[1]河南农业大学园艺学院,郑州450046 [2]中国农业科学院郑州果树研究所,郑州450009 [3]河南省苹果种质创新与利用工程研究中心,郑州450046

出　　处：《果树学报》2025年第4期693-706,共14页Journal of Fruit Science

基　　金：河南省自然科学基金青年科学基金项目(242300421568);国家自然科学基金青年科学基金项目(32402496);河南省重大科技专项(221100110400);河南省大宗水果产业技术体系(HARS-22-09-Z2);河南省中央引导地方科技发展资金项目(Z20241471014)。

摘　　要：【目的】鉴定苹果β-微管蛋白家族基因,并探究其差异表达模式,为进一步开展苹果微管蛋白的功能研究提供理论基础。【方法】基于苹果基因组数据库鉴定苹果β-微管蛋白家族成员,并对苹果β-微管蛋白家族基因进行生物信息学分析,通过实时荧光定量PCR(RT-qPCR)分析其在苹果不同砧木类型及不同组织中的表达模式。【结果】苹果基因组中有13个苹果β-微管蛋白家族成员,随机分布在11条染色体上,通过进化树分析可将其聚为5个亚组;多序列比对和基因结构分析表明苹果β-微管蛋白家族有较强的保守性;通过启动子顺式作用元件分析发现脱落酸响应元件、茉莉酸甲酯响应元件和厌氧诱导元件在苹果β-微管蛋白家族基因启动子中分布最为广泛。苹果β-微管蛋白家族基因存在一定的组织表达特异性,且MdTUB4在矮化砧木中的表达量显著低于乔化砧木,而MdTUB12表达趋势相反,表明了MdTUB4和MdTUB12可能参与调控苹果矮生。【结论】鉴定了13个β-微管蛋白家族基因,分析了其在苹果矮生中的差异表达特性,为进一步研究微管蛋白调控苹果矮生提供了理论基础。【Objective】Microtubule is composed ofα-andβ-tubulin heterodimers.It is known thatα-andβ-tubulins are encoded with large family genes and selected expression ofα-andβ-tubulin family genes plays key roles in regulating various biological processes,including plant growth and development,stress responses and signaling transduction.However,little is known about apple tubulin family genes and their potential functions.Therefore,the present study was to identify the appleβ-tubulin family genes and to determine the candidateβ-tubulin genes involved in regulating apple dwarfing.【Methods】Using Arabidopsis thalianaβ-tubulin amino acid sequences as reference,appleβ-tubulin family genes were identified from the apple reference genome GDDH13 v1.1.In the following,bioinformatic analysis was conducted to dissect the physicochemical properties,chromosome localization,phylogenetic relationships,gene structure,conserved motifs,collinearity,three-dimensional structure prediction and cis-acting elements in their promoter regions of the identified appleβ-tubulin family genes.Different tissues including mature and young leaves,xylem,phloem and stem tips of column apple Runtai No.1 were collected as materials to analyze the tissue expression patterns of appleβ-tubulin family genes through quantitative real-time PCR(RT-qPCR).To screen the candidateβ-tubulin genes involved in regulating apple dwarfing,the relative expressions of appleβ-tubulin family genes in the shoot apex of three apple dwarfing rootstocks(T337,Pamajul and JM7)and three common rootstocks(Malus prunifolia,M.micromalus and M.hupehensis)were compared by RT-qPCR.【Results】A total of 13 appleβ-tubulin sequences were identified from the apple genome.The lengths of these 13 appleβ-tubulins were from 444 aa to 450 aa,and the molecular weights ranged from 49.92 ku to 50.46 ku.The 13 appleβ-tubulin genes were randomly distributed on 11 apple chromosomes,and fragment replication events were the main factor attributed to the expansion of appleβ-tubulin

关 键 词：苹果 微管蛋白 基因家族 矮生 

分 类 号：S661.1[农业科学—果树学]