水稻纹枯病抗性相关基因OsDUF功能研究  

Functional Study of the Rice Sheath Blight Resistance Related Gene OsDUF

作　　者：王妍[1] 王梦雨 项宗敬 王奕鸣 魏松红[1] WANG Yan;WANG Mengyu;XIANG Zongjing;WANG Yiming;WEI Songhong(College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China;Longshan Science and Technology Park,Wuhu Anhui 241000,China)

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]芜湖市龙山科技园,安徽芜湖241000

出　　处：《沈阳农业大学学报》2025年第2期34-41,共8页Journal of Shenyang Agricultural University

基　　金：国家重点研发计划项目(2024YFD1201005-05);国家水稻产业技术体系项目(CARS-01);“兴辽英才计划”农业专家项目(XLYC2213046)。

摘　　要：[目的]水稻纹枯病是水稻生产中的重要病害之一,由于纹枯病菌寄主范围广泛且抗性水稻资源匮乏,给水稻生产造成严重损失。前期对盐丰47(感病)和港源8号(抗病)水稻接种纹枯病菌后转录组测序,筛选出抗病候选基因OsDUF,对其进行生物信息学分析和亚细胞定位,创制突变体材料并进行离体接种鉴定。[方法]以OsDUF为诱饵蛋白进行酵母双杂交,外源激素诱导下检测OsDUF基因表达水平变化,纹枯病菌处理下检测防卫基因及激素相关基因表达水平变化。[结果]OsDUF基因含有DUF1645结构域,其编码蛋白定位于细胞质中,预测水稻内共有10个蛋白可能与其互作。离体叶片抗性鉴定显示突变体比野生型更感病,表明OsDUF正向调控水稻抗纹枯病,一对一互作验证未发现OsDUF与预测候选蛋白互作。外源激素处理下,吲哚乙酸(IAA)、乙烯(ET)、茉莉酸(JA)使基因OsDUF上调,赤霉素(GA)、脱落酸(ABA)、水杨酸(SA)使其下调。接种纹枯病菌后,野生型水稻植株的防卫基因(OsAOS1、OsPAL1)及激素相关基因(OsERF063、LOX2)表达量上调水平均显著高于突变体。[结论]OsDUF可能通过调控防卫基因和激素信号通路参与水稻抗纹枯病。[Objective]Rice sheath blight is one of the major diseases in rice.Due to the wide host range of Rhizoctonia solani and the lack of resistant rice resources,it causes serious losses of rice production.Previous study,we investigated the roles of rice sheath blight-responsive hormones and the candidate resistance gene OsDUF,providing the theoretical basis for the prevention and controlling rice sheath blight.[Methods]The susceptible variety Yanfeng 47 and the resistant rice variety Gangyuan 8 were selected for inoculation with the rice sheath blight fungus to identify the disease resistant candidate gene OsDUF,bioinformatics analysis and subcellular localization of OsDUF were conducted.Resistance of CRISPR-Cas9-generated OsDUF mutants were compared to wildtype plants.Yeast two-hybrid assays were performed using OsDUF as bait protein,and changes in the expression levels of OsDUF genes were detected under exogenous hormonal treatments,furthermore changes in the expression levels of defence genes and hormone-related genes were detected under treatment with R.solani.[Results]Phylogenetic characterization revealed that OsDUF,encoding a cytoplasm-localized protein possessing the DUF1645 domain characteristic of stress-responsive proteins,exhibits closest evolutionary relationship to japonica rice accessions(Oryza sativa subsp.japonica).Bioinformatics interrogation further identified ten putative rice interactors through domain architecture analysis,though subsequent experimental validation remains required.Identification of resistance in isolated leaves showed that the mutant was more susceptible to the disease than the wild type,indicating that OsDUF positively regulates rice sheath blight resistance,and one-to-one interaction validation did not reveal that OsDUF interacted with the predicted candidate proteins.Under exogenous hormone treatments,the genes OsDUF were up-regulated by indoleacetic acid,ethylene,and jasmonic acid,and down-regulated by gibberellin,abscisic acid,and salicylic acid.After inoculation with R.sol

关 键 词：水稻纹枯病 OsDUF 生物信息学分析 激素 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]